Fc OxyBURST™ Green Assay Reagent
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Citations & References (14)
Invitrogen™

Fc OxyBURST™ Green Assay Reagent

The Fc OxyBURST™ Green assay reagent comprises bovine serum albumin (BSA) that has been covalently linked to dichlorodihydrofluorescein (H2DCF) andRead more
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Catalog NumberQuantity
F2902500 μL, 25 reactions
Catalog number F2902
Price (TWD)
14,280.00
온라인 행사
Ends: 31-Dec-2025
17,300.00
Save 3,020.00 (17%)
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Quantity:
500 μL, 25 reactions
Price (TWD)
14,280.00
온라인 행사
Ends: 31-Dec-2025
17,300.00
Save 3,020.00 (17%)
Each
Add to cart
The Fc OxyBURST™ Green assay reagent comprises bovine serum albumin (BSA) that has been covalently linked to dichlorodihydrofluorescein (H2DCF) and then complexed with a purified rabbit polyclonal anti-BSA antibody. When this immune complex binds to Fc receptors, the nonfluorescent H2DCF molecules are internalized within the phagovacuoloe and subsequently oxidized to green-fluorescent 2''7'-dichlorofluorescein.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration3 mg⁄ml
Detection MethodFluorescence
Dye TypeFree Radical Sensor
FormSolution
FormatTube(s), Slide(s)
Quantity500 μL, 25 reactions
Shipping ConditionWet Ice
SolubilityDMSO (Dimethylsulfoxide)
Emission488/523
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Product LineOXYBURST
Product TypeAssay Reagent
Unit SizeEach
Contents & Storage
Contains 1 vial of Fc OxyBURST™ assay green reagent (suspension in PBS containing sodium azide). Store in refrigerator (2–8°C) and protect from light.

Citations & References (14)

Citations & References
Abstract
The phosphoinositide-binding protein p40phox activates the NADPH oxidase during FcgammaIIA receptor-induced phagocytosis.
Authors:Suh CI, Stull ND, Li XJ, Tian W, Price MO, Grinstein S, Yaffe MB, Atkinson S, Dinauer MC
Journal:J Exp Med
PubMed ID:16880255
'Superoxide produced by the phagocyte reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase is essential for host defense. Enzyme activation requires translocation of p67(phox), p47(phox), and Rac-GTP to flavocytochrome b558 in phagocyte membranes. To examine the regulation of phagocytosis-induced superoxide production, flavocytochrome b558, p47(phox), p67(phox), and the FcgammaIIA receptor were expressed ... More
Severe impairment in early host defense against Listeria monocytogenes in mice deficient in acid sphingomyelinase.
Authors:Utermöhlen O, Karow U, Löhler J, Krönke M
Journal:J Immunol
PubMed ID:12594290
'The phagolysosomal compartment is crucial for the defense against infection with intracellular pathogens. Within this compartment, the TNF- and IFN-gamma-responsive acid sphingomyelinase (ASMase) generates the signaling molecule ceramide, resulting in the activation of proteases like cathepsin D. To investigate the possible role of ASMase as a mediator of the antibacterial ... More
Quantitative multiwell myeloid differentiation assay using dichlorodihydrofluorescein diacetate (H2DCF-DA) or dihydrorhodamine 123 (H2R123).
Authors:Trayner ID, Rayner AP, Freeman GE, Farzaneh F
Journal:J Immunol Methods
PubMed ID:7594627
'It is well established that the fluorescent probes dichlorodihydrofluorescein diacetate (H2DCF-DA) and dihydrorhodamine 123 (H2R123) can be used to detect the respiratory burst response of mature myeloid cells. We describe a simple, fast and quantitative assay for myeloid differentiation based on the oxidation of these probes, which can be performed ... More
A cytosolic calcium transient is not necessary for degranulation or oxidative burst in immune complex-stimulated neutrophils.
Authors:Seetoo KF, Schonhorn JE, Gewirtz AT, Zhou MJ, McMenamin ME, Delva L, Simons ER
Journal:J Leukoc Biol
PubMed ID:9307071
'Receptor-mediated activation of neutrophils (PMN) initiates possibly interdependent events, including a rapid transient increase in [Ca2+]i, implicated as a second messenger. To investigate whether this transient is required for eventual degranulation, PMN were incubated with an intracellular Ca2+ chelator (BAPTA), then exposed to chemotactic peptide [N-formyl-methionyl-leucyl-phenylalanine (fMLP)l with or without ... More
Fluorescent probes for living cells.
Authors:Johnson I
Journal:Histochem J
PubMed ID:10188922
'The functional characteristics of fluorescent probes used for imaging and measuring dynamic processes in living cells are reviewed. Initial consideration is given to general design requirements for delivery, targeting, detectability and fluorescence readout, and current technologies for attaining them. Discussion then proceeds to the more application-specific properties of intracellular ion ... More
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