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Citations & References (12)
Invitrogen™
FluoSpheres™ Carboxylate-Modified Microspheres
Achieve the brightest fluorescence with Carboxylate-Modified FluoSphere Microspheres, available in different colors and particle sizes.
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| Catalog Number | Diameter (Metric) | Color | Excitation/Emission | Quantity |
|---|---|---|---|---|
| F8813 | 0.5 μm | Yellow-Green | 505/515 nm | 10 mL |
| F8803 | 0.1 μm | Yellow-Green | 505/515 nm | 10 mL |
| F8816 | 1.0 μm | Crimson | 625/645 nm | 2 mL |
| F8823 | 1.0 μm | Yellow-Green | 505/515 nm | 10 mL |
| F8801 | 0.1 μm | Red | 580/605 nm | 10 mL |
| F8811 | 0.2 μm | Yellow-Green | 505/515 nm | 10 mL |
| F8807 | 0.2 μm | Dark Red | 660/680 nm | 2 mL |
| F10720 | 0.04 μm | Yellow-Green, Orange, Red, Dark Red | 505/515, 540/560, 580/605, 660/680 nm | 1 mL/each |
| F20881 | 0.2 μm | Orange | 365/610 nm | 2 mL |
| F8783 also known as F-8783 | 0.02 μm | Dark Red | 660/680 nm | 2 mL |
| F8786 | 0.02 μm | Red | 580/605 nm | 10 mL |
| F8795 also known as F-8795 | 0.04 μm | Yellow-Green | 505/515 nm | 1 mL |
| F8820 | 1.0 μm | Orange | 540/560 nm | 10 mL |
| F8825 | 2.0 μm | Nile Red | 535/575 nm | 2 mL |
| F8827 | 2.0 μm | Yellow-Green | 505/515 nm | 2 mL |
| F8781 also known as F-8781 | 0.02 μm | Blue | 365/415 nm | 10 mL |
| F8782 also known as F-8782 | 0.02 μm | Crimson | 625/645 nm | 2 mL |
| F8784 | 0.02 μm | Nile Red | 535/575 nm | 10 mL |
| F8787 | 0.02 μm | Yellow-Green | 505/515 nm | 10 mL |
| F8789 also known as F-8789 | 0.04 μm | Dark Red | 660/680 nm | 1 mL |
| F8792 also known as F-8792 | 0.04 μm | Orange | 540/560 nm | 1 mL |
| F8793 | 0.04 μm | Red | 580/605 nm | 1 mL |
| F8794 | 0.04 μm | Red-Orange | 565/580 nm | 1 mL |
| F8797 | 0.1 μm | Blue | 350/440 nm | 10 mL |
| F8799 | 0.1 μm | Infrared | 715/755 nm | 1 mL |
| F8800 also known as F-8800 | 0.1 μm | Orange | 540/560 nm | 10 mL |
| F8805 | 0.2 μm | Blue | 365/415 nm | 10 mL |
| F8806 | 0.2 μm | Crimson | 625/645 nm | 2 mL |
| F8809 | 0.2 μm | Orange | 540/560 nm | 10 mL |
| F8810 | 0.2 μm | Red | 580/605 nm | 10 mL |
| F8812 | 0.5 μm | Red | 580/605 nm | 10 mL |
| F8814 | 1.0 μm | Blue | 365/415 nm | 10 mL |
| F8815 | 1.0 μm | Blue | 350/440 nm | 10 mL |
| F8819 | 1.0 μm | Nile Red | 535/575 nm | 10 mL |
| F8821 | 1.0 μm | Red | 580/605 nm | 10 mL |
| F8824 also known as F-8824 | 2.0 μm | Blue | 365/415 nm | 2 mL |
| F8826 | 2.0 μm | Red | 580/605 nm | 2 mL |
Catalog number F8813
Price (TWD)
15,120.00
Online offer
Ends: 31-Dec-2025
21,600.00Save 6,480.00 (30%)
Each
Diameter (Metric):
0.5 μm
Color:
Yellow-Green
Excitation/Emission:
505/515 nm
Quantity:
10 mL
Price (TWD)
15,120.00
Online offer
Ends: 31-Dec-2025
21,600.00Save 6,480.00 (30%)
Each
Easily perform flow cytometry, microscopy, HTS, HCS, immunoassay, and other laboratory applications using our extensive selection of FluoSpheres Carboxylate-Modified Microspheres. FluoSphere beads can be used in passive adsorption or active, covalent coupling of proteins, nucleic acids, and biomolecules for particle capture applications. FluoSphere microspheres are loaded with proprietary fluorescent dyes, making them the brightest microspheres available.
Visualize the brightest fluorescence for laboratoy applications including fluorescence microscopy, flow cytometry, HTS, HCS, and cell tracing with our Carboxylate-Modified FluoSphere Microspheres, which are manufactured from polystyrene microspheres and loaded with different proprietary dyes. Using specialized staining methods enables all of the fluorescent dye molecules to be contained inside each polystyrene microsphere instead of on the bead's surface. This protective environment within the bead shields the dye from detrimental environmental effects, such as photobleaching. Our carboxylate-modified microspheres are coated with a hydrophilic polymer containing multiple carboxylic acids for covalent attachment of ligands. A range of particle sizes is available for different research uses and experiments.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Excitation/Emission505/515 nm
Product LineFLUOSPHERES
Quantity10 mL
Surface ModificationCarboxylate
ColorYellow-Green
Diameter (Metric)0.5 μm
For Use With (Application)Fluorescence Microscopy
MaterialPolystyrene
Product TypeCarboxylate-Modified Microsphere
Unit SizeEach
Contents & Storage
Store in refrigerator (2–8°C) and protect from light.
Frequently asked questions (FAQs)
I have some FluoSpheres polystyrene microspheres, with 20 nm diameter. They are aggregating a lot. What can I do about it?
I sonicated my 2.0 µm carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?
What is the warranty for FluoSpheres microspheres?
After washing and centrifugation, there was only a very small pellet left of my microsphere beads and the solution was transparent. Why is this?
I've had my microspheres for over a year, and I'm wondering if they're still good to use. What are some good ways to check their functionality?
Citations & References (12)
Citations & References
Abstract
Altered membrane dynamics of quantum dot-conjugated integrins during osteogenic differentiation of human bone marrow derived progenitor cells.
Journal:Biophysical journal
PubMed ID:17114225
Functionalized quantum dots offer several advantages for tracking the motion of individual molecules on the cell surface, including selective binding, precise optical identification of cell surface molecules, and detailed examination of the molecular motion without photobleaching. We have used quantum dots conjugated with integrin antibodies and performed studies to quantitatively
Quantitating intracellular transport of polyplexes by spatio-temporal image correlation spectroscopy.
Journal:Proc Natl Acad Sci U S A
PubMed ID:15897455
'Quantitatively understanding how nonviral gene delivery vectors (polyplexes) are transported inside cells is essential before they can be optimized for gene therapy and medical applications. In this study, we used spatio-temporal image correlation spectroscopy (ICS) to follow polymer-nucleic acid particles (polyplexes) of various sizes and analyze their diffusive-like and flow
The photon counting histogram in fluorescence fluctuation spectroscopy.
Journal:Biophys J
PubMed ID:10388780
'Fluorescence correlation spectroscopy (FCS) is generally used to obtain information about the number of fluorescent particles in a small volume and the diffusion coefficient from the autocorrelation function of the fluorescence signal. Here we demonstrate that photon counting histogram (PCH) analysis constitutes a novel tool for extracting quantities from fluorescence
Thermal fluctuations of grafted microtubules provide evidence of a length-dependent persistence length.
Journal:Proc Natl Acad Sci U S A
PubMed ID:16801537
'Microtubules are hollow cylindrical structures that constitute one of the three major classes of cytoskeletal filaments. On the mesoscopic length scale of a cell, their material properties are characterized by a single stiffness parameter, the persistence length l(p). Its value, in general, depends on the microscopic interactions between the constituent
Why molecules move along a temperature gradient.
Journal:Proc Natl Acad Sci U S A
PubMed ID:17164337
'Molecules drift along temperature gradients, an effect called thermophoresis, the Soret effect, or thermodiffusion. In liquids, its theoretical foundation is the subject of a long-standing debate. By using an all-optical microfluidic fluorescence method, we present experimental results for DNA and polystyrene beads over a large range of particle sizes, salt