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Citations & References (32)
Invitrogen™
ImaGene Green™ C12FDG lacZ Gene Expression Kit
The ImaGene Green™ lacZ Gene Expression Kit contains a fluorescein-based galactosidase substrate that has been covalently modified to include aRead more
| Catalog Number | Quantity |
|---|---|
| I2904 | 1 kit |
Catalog number I2904
Price (TWD)
22,330.00
Online offer
Ends: 31-Dec-2025
31,900.00Save 9,570.00 (30%)
Each
Quantity:
1 kit
Price (TWD)
22,330.00
Online offer
Ends: 31-Dec-2025
31,900.00Save 9,570.00 (30%)
Each
The ImaGene Green™ lacZ Gene Expression Kit contains a fluorescein-based galactosidase substrate that has been covalently modified to include a 12-carbon lipophilic moiety, C12FDG. Once inside the cell, the substrates are cleaved by β-galactosidase, producing a fluorescent product that is well retained by the cells, probably by incorporation of the lipophilic tail within the cellular membrane. In addition to the C12FDG, the kits also include the broad-spectrum β-galactosidase inhibitor, PETG, and chloroquine diphosphate for inhibiting acidic hydrolysis of the substrate.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Assayβ-Gal (lacZ) Assay
Detection MethodFluorescence
Product LineImaGene Green
Product Typeβ-Galactosidase Reporter Gene Assay System
Quantity1 kit
Shipping ConditionRoom Temperature
SubstrateC12FDG
Substrate PropertiesChemical Substrate
Substrate TypeBeta-Gal Substrate
Target EnzymeBeta-Galactosidase
FormatKit
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -30°C) and protect from light.
Citations & References (32)
Citations & References
Abstract
Rapid flow cytometric method for measuring senescence associated beta-galactosidase activity in human fibroblasts.
Journal:Cytometry A
PubMed ID:19777541
'Senescence associated-beta-galactosidase (SA-beta-gal) activity is a widely used marker for cellular senenescence. SA-beta-gal activity is routinely detected cytochemically, manually discriminating negative from positive cells. This method is time-consuming, subjective and therefore prone to operator-error. We aimed to optimize a flow cytometric method described by other workers using endothelial cells to
Optical imaging fiber-based single live cell arrays: a high-density cell assay platform.
Journal:Anal Chem
PubMed ID:12141663
'A high-density, ordered array containing thousands of microwells is fabricated on an optical imaging fiber. Each individually addressable microwell is used to accommodate a single living cell. A charged coupled device (CCD) detector is employed to monitor and spatially resolve the fluorescence signals obtained from each individual cell, allowing simultaneous
An optimized electroporation protocol applicable to a wide range of cell lines.
Journal:Biotechniques
PubMed ID:7873174
'A number of transfection methods for mammalian cells are available; however, many cell lines may appear resistant to efficient transfection, or at best, necessitate lengthy optimization procedures in recommended protocols. We describe here an electroporation protocol that yields highly efficient gene transfer (20%-100% of surviving cells) in all 19 cell
Mapping mechanisms and charting the time course of premature cell senescence and apoptosis: lysosomal dysfunction and ganglioside accumulation in endothelial cells.
Journal:Am J Physiol Renal Physiol
PubMed ID:17928415
'Endothelial cells subjected to glycated collagen I develop premature senescence within 3-5 days, as revealed by increased senescence-associated beta-galactosidase activity, decreased proliferation, and an increase in cell size. Here, we analyzed the time course and possible mechanisms of this process. Lysosomal integrity studies revealed a rapid collapse of pH gradient
Use of fluorescence-activated cell sorting for rapid isolation of insect cells harboring recombinant baculovirus.
Journal:Methods Cell Biol
PubMed ID:7877509