Spectinomycin dihydrochloride pentahydrate, Cell Culture Grade

Name: Spectinomycin dihydrochloride pentahydrate, Cell Culture Grade
SKU: J61820.14

Spectinomycin dihydrochloride pentahydrate, CAS # 22189-32-8, is the hydrochloride salt form of spectinomycin, an aminocyclitol compound showing antibacterial and antimicrobial activities. | CAS: 22189-32-8 | C14H36Cl2N2O12 | 495.34 g/mol

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25 g

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Catalog number J61820.14

also known as J61820-14

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Quantity:

25 g

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Chemical Identifiers

CAS22189-32-8

IUPAC Namedihydrogen (1R,3S,5R,8R,10R,11S,12S,13R,14S)-8,12,14-trihydroxy-5-methyl-11,13-bis(methylamino)-2,4,9-trioxatricyclo[8.4.0.0³,⁸]tetradecan-7-one pentahydrate dichloride

Molecular FormulaC14H36Cl2N2O12

InChI KeyDCHJOVNPPSBWHK-UXXUFHFZSA-N

SMILES[H+].[H+].O.O.O.O.O.[Cl-].[Cl-].CN[C@@H]1[C@H](O)[C@H](NC)[C@H]2O[C@]3(O)[C@@H](O[C@H](C)CC3=O)O[C@@H]2[C@H]1O

Specifications

Specification Sheet

pH3.5-6.0

FormSlightly hygroscopic powder

Appearance (Color)White to cream

Potency≥ 603 IU/mg

Water Content (Karl Fischer Titration)16.0-20.0%

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Suitable for battery materials development.

General Description

Spectinomycin dihydrochloride pentahydrate is derived from Streptomyces spectabilis and presents antibacterial and antimicrobial activities
Spectinomycin hydrochloride is a compound that inhibits the 30S ribosomal subunit of bacteria and interrupts protein synthesis and peptide elongation, leading to bacterial cell death

Application

Spectinomycin dihydrochloride pentahydrate is a compound that is active against gram-negative bacteria
In the laboratory, this compound is used in cell culture applications
It prevents contamination and can be used to select for resistant bacteria and plant cells containing the marker gene Spcr
It has been used to study respiratory tract infections caused by Pasteurella multocida and Mannheimia haemolytica
In genetic approaches, this compound can be used to amplify the plasmid copy number

RUO – Research Use Only

General References:

Korte, H. L.; Saini, A.; Trotter, V. V.; Butland, G. P.; Arkin, A. P.; Wall, J. D. Independence of nitrate and nitrite inhibition of Desulfovibrio vulgaris Hildenborough and use of nitrite as a substrate for growth. Environ. Sci. Technol. 2015, 49 (2), 924-931.
Pernil, R.; Picossi, S.; Herrero, A.; Flores, E.; Mariscal, V. Amino Acid Transporters and Release of Hydrophobic Amino Acids in the Heterocyst-Forming Cyanobacterium Anabaena sp. Strain PCC 7120. Life 2015, 5 (2), 1282-1300.