Phenol:Chloroform:Isoamyl Alcohol (25:24:1), Molecular Biology Grade, Ultrapure

Name: Phenol:Chloroform:Isoamyl Alcohol (25:24:1), Molecular Biology Grade, Ultrapure
SKU: J75831.AN

Nucleic acid extraction and purification

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100 mL

400 mL

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Catalog number J75831.AN

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Quantity:

400 mL

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Specifications

Chemical Name or MaterialPhenol:Chloroform:Isoamyl Alcohol (25:24:1)

DOT InformationHazard Class: 8; Packaging Group: II

Health Hazard 1H301+H311+H331-H314-H335-H341-H350-H361d-H372

Health Hazard 3P201-P202-P260-P264b-P270-P271-P281-P303+P361+P353-P304+P340-P305+P351+P338-P310-P330-P331-P363-P501c

PackagingPlastic bottle

Nucleic acid extraction and purification.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

RUO – Research Use Only

Frequently asked questions (FAQs)

How should I store Phenol:Chloroform:Isoamyl Alcohol (25:24:1), Molecular Biology Grade, Ultrapure (Cat. Nos. J75831.AE, J75831.AN)?

Phenol:Chloroform:Isoamyl Alcohol (25:24:1), Molecular Biology Grade, Ultrapure should be stored at 4 degrees C.

Do you have instructions for RNA isolation with Phenol:Chloroform:Isoamyl Alcohol (25:24:1), Molecular Biology Grade, Ultrapure (Cat. Nos. J75831.AE, J75831.AN)?

We recommend the following Phenol/Chloroform RNA Isolation Method:
A. Guanidine HCI Homogenization Buffer for RNA Isolation: 8.0M Guanidine HCI, 0.1M Sodium Acetate, pH 5.5, 5.0mM Dithiothreitol, 0.5% Sodium Lauryl Sarcosinate
B. Extraction with Phenol: Most extractions are performed using equal volumes of equilibrated phenol with respect to the sample volume. Normally, the extraction is performed twice with phenol and once with a 1:1 solution of phenol/chloroform. Hence, it is estimated that 25 ml of phenol is required for every 10 ml of sample. Note: If regular phenol is purchased, it will need to be equilibrated before use. For this reason, many customers may prefer to purchase phenol from USB that is already equilibrated (Cat. No. J75829).
C. RNA Resuspension Buffer: TE, pH 7.5

I received my order of Phenol:Chloroform:Isoamyl Alcohol (25:24:1), Molecular Biology Grade, Ultrapure (Cat. Nos. J75831.AE, J75831.AN) and the pH is 6.7, but the product page says the pH should be 8.0. Why is that?

Phenol:Chloroform:lsoamyl Alcohol (25:24:1) is packaged at pH 6.7, the product is accompanied by a separate Alkaline Equilibration Buffer. When ready to use add the supplemental buffer's entire contents to the Phenol:Chloroform:lsoamyl Alcohol, then mix or shake, and allow to separate. Once this is completed the product will be at a pH of 8.0.