Qdot™ 605 ITK™ Amino (PEG) Quantum Dots
Qdot™ 605 ITK™ Amino (PEG) Quantum Dots
Invitrogen™

Qdot™ 605 ITK™ Amino (PEG) Quantum Dots

Qdot™ 605 ITK™ amino (PEG) quantum dots are the ideal starting material for preparing custom conjugates of ultrabright and photostableRead more
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Catalog NumberQuantity
Q21501MP250 μL
Catalog number Q21501MP
Price (TWD)
27,580.00
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Ends: 31-Dec-2025
39,400.00
Save 11,820.00 (30%)
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Quantity:
250 μL
Price (TWD)
27,580.00
Online offer
Ends: 31-Dec-2025
39,400.00
Save 11,820.00 (30%)
Each
Add to cart
Qdot™ 605 ITK™ amino (PEG) quantum dots are the ideal starting material for preparing custom conjugates of ultrabright and photostable fluorescently labeled proteins or other biopolymers. These probes are functionalized with amine-derivatized PEG, which prevents non-specific interactions and provides a convenient handle for conjugation. The amino quantum dots react efficiently with isothiocyanates and succinimidyl esters, or with native carboxylic acids using water-soluble carbodiimides such as EDC. Such derivatives may be used for various labeling and tracking applications that require ultrabright and stable fluorescence. Our Qdot™ ITK™ amino quantum dots are provided as 8 μM solutions and are available in 8 colors of Qdot™ probes.

Important Features of Qdot™ ITK™ Amino Quantum Dots:
• Qdot™ 605 ITK™ amino quantum dot has emission maxima of ∼605 nm
• Extremely photostable and bright fluorescence
• Efficiently excited with single-line excitation sources
• Narrow emission, large stokes shift
• Available in multiple colors
• Ideal for various labeling and tracking applications


Properties of Qdot™ Nanocrystals
Qdot™ probes are ideal for imaging and labeling applications that require bright fluorescent signals and/or real-time tracking. Unique among fluorescent reagents, all nine available colors of Qdot™ probes can be simultaneously excited with a single (UV to blue-green) light source. This property makes these reagents excellent for economical and user-friendly multiplexing applications. Qdot™ labels are based on semiconductor nanotechnology and are similar in scale to moderately sized proteins.

About the Innovator’s Tool Kit Qdot™ ITK™ Reagents
These Qdot™ ITK™ probes are ideal for researchers who wish to prepare specific (non-stocked) conjugates for their applications and need customizable conjugation functionality.

Other Forms of Qdot™ Nanocrystals are Available
In addition to the amine-derivatized form, we offer Qdot™ ITK™ quantum dots with carboxyl and aliphatic hydrocarbon modifications. We’ve also developed a wide range of Qdot™ nanocrystals conjugates and labeling kits. Investigate the properties of Qdot™ nanocrystals or read the Molecular Probes™ Handbook Section 6.6—Qdot™ Nanocrystals to find out more.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical ReactivityCarboxylic Acid, Ketone, Aldehyde
Concentration8 μM
Emission605
Label or DyeQdot™ 605
Product TypeQuantum Dot
Quantity250 μL
Reactive MoietyAmine, Primary Amine
Shipping ConditionRoom Temperature
Label TypeQdot Nanocrystals
Product LineITK, Qdot
Unit SizeEach
Contents & Storage
Store in refrigerator (2–8°C).

Frequently asked questions (FAQs)

How large are the Qdot nanocrystals?

The core/shells are only a few nanometers in diameter (some are elliptical), but with the outer polymer coatings, a fully-functionalized Qdot nanocrystal can range from 15 to 21 nm in hydrodynamic diameter, similar in size to some proteins.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the best way to remove white precipitate from my ITK Qdot nanocrystals?

Spinning your ITK Qdot nanocrystals at approximately 3,000 rpm for 3-5 minutes should remove the white precipitate from the supernatant. Use the supernatant immediately.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I see a white precipitate in my ITK Qdot nanocrystals; should I be concerned?

The precipitate in the organic ITK Qdot nanocrystals occurs with some frequency. The ITK Qdot nanocrystals sometimes include impurities that show as a white precipitate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Why do my Qdot nanocrystals appear to be blinking?

Blinking is an inherent property of quantum dots; in fact, all single-luminescent molecules blink, including organic dyes. The brightness and photostability of Qdot nanocrystals makes the blinking more visibly apparent. Under higher energy excitation, Qdot nanocrystals blink even faster.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

My Qdot nanocrystals were brightly fluorescent before I mounted my samples; now I'm seeing a loss of fluorescence. Why is this happening?

Appropriate mounting media selection is very important to retain the fluorescence of Qdot nanocrystals. In our studies, Qdot nanocrystals work best with the following mountants:

HistoMount medium (Cat No. 00-8030); best for long term archiving
Cytoseal 60 Mountant
Clarion Mountant
Most polyvinyl alcohol-based mountants (limited storage time, less than weeks)
Water-based mountants (limited storage time, less than week)
Up to 50% glycerol (limited storage time, less than week)
Note: We do not recommend using ProLong mounting media with Qdot nanocrystals as it will quench their fluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (5)

Citations & References
Abstract
Development of homogeneous binding assays based on fluorescence resonance energy transfer between quantum dots and Alexa Fluor fluorophores.
Authors:Nikiforov TT, Beechem JM
Journal:Anal Biochem
PubMed ID:16860286
'We studied the fluorescence resonance energy transfer (FRET) between quantum dots emitting at 565, 605, and 655 nm as energy donors and Alexa Fluor fluorophores with absorbance maxima at 594, 633, 647, and 680 nm as energy acceptors. As a first step, we prepared covalent conjugates between all three types ... More
Quantum dot targeting with lipoic acid ligase and HaloTag for single-molecule imaging on living cells.
Authors:Liu DS, Phipps WS, Loh KH, Howarth M, Ting AY,
Journal:ACS Nano
PubMed ID:23181687
'We present a methodology for targeting quantum dots to specific proteins on living cells in two steps. In the first step, Escherichia coli lipoic acid ligase (LplA) site-specifically attaches 10-bromodecanoic acid onto a 13 amino acid recognition sequence that is genetically fused to a protein of interest. In the second ... More
Quantum dots provide an optical signal specific to full collapse fusion of synaptic vesicles.
Authors:Zhang Q, Cao YQ, Tsien RW,
Journal:Proc Natl Acad Sci U S A
PubMed ID:17968015
Synaptic vesicles are responsible for releasing neurotransmitters and are thus essential to brain function. The classical mode of vesicle recycling includes full collapse of the vesicle into the plasma membrane and clathrin-mediated regeneration of a new vesicle. In contrast, a nonclassical mode known as  ... More
Novel system to achieve one-pot modification of cargo molecules with oligoarginine vectors for intracellular delivery.
Authors:Takayama K, Tadokoro A, Pujals S, Nakase I, Giralt E, Futaki S,
Journal:Bioconjug Chem
PubMed ID:19161253
There is a growing number of reports showing the usefulness of cell-penetrating peptides (CPPs) including oligoarginines for intracellular delivery of macromolecules. Although the covalent attachment of the CPP segments to the cargo molecules is usually required to ensure effective delivery, conventional methods of conjugation need several manipulation steps that are ... More
Optical coding of mammalian cells using semiconductor quantum dots.
Authors:Mattheakis LC, Dias JM, Choi YJ, Gong J, Bruchez MP, Liu J, Wang E
Journal:Anal Biochem
PubMed ID:15051536
Cell-based assays are widely used to screen compounds and study complex phenotypes. Few methods exist, however, for multiplexing cellular assays or labeling individual cells in a mixed cell population. We developed a generic encoding method for cells that is based on peptide-mediated delivery of quantum dots (QDs) into live cells. ... More