Quant-it™ RiboGreen Reagent and RNA Assay Kit
For accurate quantification and a streamlined workflow try our new Quant-iT RiboGreen ReadyPlates!
Quant-it™ RiboGreen Reagent and RNA Assay Kit
Citations & References (18)
Invitrogen™

Quant-it™ RiboGreen Reagent and RNA Assay Kit

Quant-iT RiboGreen RNA Reagent is an ultrasensitive fluorescent nucleic acid stain for quantitating RNA in solution. The Quant-iT RiboGreen RNA Assay Kit comes with reagent, dilution buffer, and rRNA standards to provide a linear detection range of 1–200 ng of RNA.
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Catalog NumberQuantityProduct TypeQuantitation Range
R327001 kitRediPlate 96 RiboGreen RNA Kit3 to 200 ng
R114901 kitQuant-iT RiboGreen RNA Kit1 to 200 ng
R114911 mLQuant-iT RiboGreen RNA Reagent1 to 200 ng
Catalog number R32700
Price (TWD)
17,150.00
Online offer
Ends: 31-Dec-2025
24,500.00
Save 7,350.00 (30%)
Each
Add to cart
Quantity:
1 kit
Product Type:
RediPlate 96 RiboGreen RNA Kit
Quantitation Range:
3 to 200 ng
Price (TWD)
17,150.00
Online offer
Ends: 31-Dec-2025
24,500.00
Save 7,350.00 (30%)
Each
Add to cart
Quant-iT RiboGreen RNA Reagent is an ultrasensitive fluorescent nucleic acid stain for quantitating RNA in solution. The Quant-iT RiboGreen RNA Assay Kit comes with reagent, dilution buffer, and rRNA standards to provide a linear detection range of 1–200 ng of RNA.

Workflow 

This easy-to-use reagent offers two detection ranges: a high-assay range and a low-assay range for sensitive and broad range detection.  To use, simply prepare the working solution by diluting the provided dye with TE-Buffer, then aliquotting 100 µL of the working solution with standards and samples for a total reaction volume of 200 µL.  RNA is then quantitated using a fluorescence microplate reader (such as Varioskan ALF) at standard fluorescein wavelengths.

Applications

Detecting and quantitating small amounts of RNA is important in many applications including measuring yields of in vitro transcribed RNA and measuring RNA concentrations before performing Northern blot analyses, S1 nuclease assays, RNase protection assays, cDNA library preparation, reverse transcription PCR, and differential display PCR.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Excitation/Emission500/525
For Use With (Equipment)Microplate Reader
Product LineRIBOGREEN, RediPlate
Product TypeRediPlate 96 RiboGreen RNA Kit
Quantitation Range3 to 200 ng
Quantity1 kit
Shipping ConditionRoom Temperature
Detection MethodFluorescence
Unit SizeEach

Frequently asked questions (FAQs)

When I try to run RediPlate 96 RiboGreen RNA Kit (Cat. No. R32700) on my Varioskan LUX instrument, it says there is no plate. What should I do?

When using a strip plate with the Varioskan LUX plate reader, ensure that there is a strip covering the D6 well, as this is where the instrument checks for the presence of a plate. The strip does not need to be active; it just needs to be in place for the instrument to verify the plate's presence. Alternatively, you can resolve this issue by adjusting the software settings. Go to the general settings and uncheck the box labeled "Check plate and tip prime vessel before session execution." This will immediately solve the problem.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Does the Quant-it RiboGreen RNA Assay Kit and RiboGreen RNA Reagent (Cat. No. R32700) work with double-stranded RNA (dsRNA)?

We have not validated measurement of dsRNA with the Quant-it RiboGreen RNA Assay Kit and RiboGreen RNA Reagent (Cat. No. R32700). If you are using RiboGreen dye to measure dsRNA, then you should use your own dsRNA standards, rather than the standards provided with the kit, to get an accurate measurement.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center

Citations & References (18)

Citations & References
Abstract
A fluorescence-based assay for multisubunit DNA-dependent RNA polymerases.
Authors:Kuhlman P, Duff HL, Galant A
Journal:Anal Biochem
PubMed ID:14690681
The properties of DNA-dependent RNA polymerases have been studied since the 1960s, but considerable interest in probing RNA polymerase structure/function relationships, the roles of different classes of RNA polymerases in cellular processes, and the feasibility of using RNA polymerases as drug targets still exists. Historically, RNA polymerase activity has been ... More
Quantification of enterovirus RNA in sludge samples using single tube real-time RT-PCR.
Authors:Monpoeho S, Dehée A, Mignotte B, Schwartzbrod L, Marechal V, Nicolas JC, Billaudel S, Férré V
Journal:Biotechniques
PubMed ID:10907082
'We have developed a quantitative RT-PCR method that can be used to determine the amount of enterovirus RNA in urban sludge samples. This method combines Taq-Man technology with the ABI Prism 7700 real-time sequence detection system. We optimized a one-step RT-PCR that uses a dual-labeled fluorogenic probe to quantify the ... More
mRNA expression profiling of laser microbeam microdissected cells from slender embryonic structures.
Authors:Scheidl SJ, Nilsson S, Kalén M, Hellström M, Takemoto M, Håkansson J, Lindahl P
Journal:Am J Pathol
PubMed ID:11891179
'Microarray hybridization has rapidly evolved as an important tool for genomic studies and studies of gene regulation at the transcriptome level. Expression profiles from homogenous samples such as yeast and mammalian cell cultures are currently extending our understanding of biology, whereas analyses of multicellular organisms are more difficult because of ... More
A mitotic cascade of NIMA family kinases. Nercc1/Nek9 activates the Nek6 and Nek7 kinases.
Authors:Belham C, Roig J, Caldwell JA, Aoyama Y, Kemp BE, Comb M, Avruch J
Journal:J Biol Chem
PubMed ID:12840024
'The Nek family of protein kinases in humans is composed of 11 members that share an amino-terminal catalytic domain related to NIMA, an Aspergillus kinase involved in the control of several aspects of mitosis, and divergent carboxyl-terminal tails of varying length. Nek6 (314AA) and Nek7 (303AA), 76% identical, have little ... More
Quantification of cDNA generated by reverse transcription of total RNA provides a simple alternative tool for quantitative RT-PCR normalization.
Authors:Libus J, Storchová H
Journal:Biotechniques
PubMed ID:16925017
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