Streptavidin, Alexa Fluor™ 594 Conjugate

Citations & References (41)

Invitrogen™

Streptavidin, Alexa Fluor™ 594 Conjugate

Name: Streptavidin, Alexa Fluor™ 594 Conjugate
SKU: S11227
Price: 11520.00 TWD

Alexa Fluor™ 594 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor™ dye). Streptavidin hasRead more

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Catalog Number	Quantity	Form
S11227	1 mg	Solid
S32356	0.5 mL	Liquid

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Catalog number S11227

Price (TWD)

11,520.00

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Ends: 31-Dec-2025

14,400.00

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Quantity:

1 mg

Form:

Solid

Price (TWD)

11,520.00

Online offer

Ends: 31-Dec-2025

14,400.00

Save 2,880.00 (20%)

Each

Add to cart

Alexa Fluor™ 594 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor™ dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor™ dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.

Important Features of Alexa Fluor™ 594 Streptavidin Conjugates:
• Alexa Fluor™ 594 streptavidin conjugate has Ex/Em maxima of ∼ (590/617)
• Bright, photostable fluorescence
• High solubility in aqueous solutions
• Available in multiple colors
• Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

Properties of Alexa Fluor™ Dyes
Alexa Fluor™ dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links:

Learn more about Avidin-Biotin Detection

Learn more about Alexa Fluor™ Dyes

Find out about other Labeled Streptavidin Conjugates

Read Avidin and Streptavidin Conjugates-Section 7.6 in the Molecular Probes Handbook

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Label or DyeAlexa Fluor Dyes

Product TypeStreptavidin Conjugate (fluorescent)

Quantity1 mg

Shipping ConditionRoom Temperature

ConjugateAlexa Fluor 594

FormSolid

Product LineAlexa Fluor

Unit SizeEach

Contents & Storage

Store in freezer (-5 to -30°C) and protect from light.

Frequently asked questions (FAQs)

I am planning to use a fluorescent streptavidin labeled conjugate. What are the storage conditions and shelf life for the lyophilized powder and reconstituted solution?

In the lyophilized powder form, the fluorescent streptavidin labeled conjugate is stable for six months when stored at -20 degrees C, desiccated, and protected from light. The reconstituted solution is stable for approximately six months when stored at 4 degrees C, protected from light, with the addition of sodium azide to a final concentration of 5 mM or thimerosal to 0.2 mM. For longer storage, we recommend dividing the solution into aliquots and freezing at -20 degrees C, protected from light. Avoid repeated freezing and thawing of the solution.

I am planning to use a fluorescent streptavidin labeled conjugate. How should I prepare the working solution of the conjugate?

The fluorescent streptavidin labeled conjugate solution can be made by dissolving the powder in 0.5-1.0 mL of PBS or other suitable buffer. For details, please refer to page 4 of the "Streptavidin and Fluorescent Conjugates of Streptavidin" manual (https://assets.fishersci.com/TFS-Assets/LSG/manuals/mp00888.pdf).

Citations & References (41)

Citations & References

Abstract

Avian flu: influenza virus receptors in the human airway.

Authors:Shinya K,Ebina M,Yamada S,Ono M,Kasai N,Kawaoka Y

Journal:Nature

PubMed ID:16554799

The membrane-bound histidine acid phosphatase TbMBAP1 is essential for endocytosis and membrane recycling in Trypanosoma brucei.

Authors:Engstler M, Weise F, Bopp K, Grünfelder CG, Günzel M, Heddergott N, Overath P

Journal:J Cell Sci

PubMed ID:15855239

'In the parasitic protozoan Trypanosoma brucei, endocytosis and exocytosis occur exclusively at an invagination of the plasma membrane around the base of the flagellum, called the flagellar pocket, which actively communicates by vesicular membrane flow with cisternal/tubulovesicular endosomes. The division of the cell surface into three morphologically distinct sub-domains and ... More

Cyclin I protects podocytes from apoptosis.

Authors:Griffin SV, Olivier JP, Pippin JW, Roberts JM, Shankland SJ

Journal:J Biol Chem

PubMed ID:16847066

'The limited regenerative capacity of the glomerular podocyte following injury underlies the development of glomerulosclerosis and progressive renal failure in a diverse range of kidney diseases. We discovered that, in the kidney, cyclin I is uniquely expressed in the glomerular podocyte, and have constructed cyclin I knock-out mice to explore ... More

Identification of nicotinic acetylcholine receptor recycling and its role in maintaining receptor density at the neuromuscular junction in vivo.

Authors:Bruneau E, Sutter D, Hume RI, Akaaboune M

Journal:J Neurosci

PubMed ID:16251443

'In the CNS, receptor recycling is critical for synaptic plasticity; however, the recycling of receptors has never been observed at peripheral synapses. Using a novel imaging technique, we show here that nicotinic acetylcholine receptors (AChRs) recycle into the postsynaptic membrane of the neuromuscular junction. By sequentially labeling AChRs with biotin-bungarotoxin ... More

Development of homogeneous binding assays based on fluorescence resonance energy transfer between quantum dots and Alexa Fluor fluorophores.

Authors:Nikiforov TT, Beechem JM

Journal:Anal Biochem

PubMed ID:16860286

'We studied the fluorescence resonance energy transfer (FRET) between quantum dots emitting at 565, 605, and 655 nm as energy donors and Alexa Fluor fluorophores with absorbance maxima at 594, 633, 647, and 680 nm as energy acceptors. As a first step, we prepared covalent conjugates between all three types ... More

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