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Citations & References (9)
Invitrogen™
Streptavidin, Alexa Fluor™ 350 conjugate
Alexa Fluor™ 350 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor™ dye). Streptavidin hasRead more
| Catalog Number | Quantity |
|---|---|
| S11249 | 1 mg |
Catalog number S11249
Price (TWD)
10,430.00
Online offer
Ends: 31-Dec-2025
14,900.00Save 4,470.00 (30%)
Each
Quantity:
1 mg
Price (TWD)
10,430.00
Online offer
Ends: 31-Dec-2025
14,900.00Save 4,470.00 (30%)
Each
Alexa Fluor™ 350 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor™ dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor™ dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.
Important Features of Alexa Fluor™ 350 Streptavidin Conjugates:
• Alexa Fluor™ 350 streptavidin conjugate has Ex/Em maxima of ∼ (346/442)
• Bright, photostable fluorescence
• High solubility in aqueous solutions
• Available in multiple colors
• Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more
Properties of Alexa Fluor™ Dyes
Alexa Fluor™ dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.
Consult user Manual for solubility instructions.
Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.
Related Links:
Learn more about Avidin-Biotin Detection
Learn more about Alexa Fluor™ Dyes
Find out about other Labeled Streptavidin Conjugates
Read Avidin and Streptavidin Conjugates-Section 7.6 in the Molecular Probes Handbook
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Label or DyeAlexa Fluor
Product TypeStreptavidin Conjugate (fluorescent)
Quantity1 mg
Shipping ConditionRoom Temperature
ConjugateAlexa Fluor 350
FormSolid
Product LineAlexa Fluor
Unit SizeEach
Contents & Storage
Store in freezer (-5°C to -30°C) and protect from light.
Citations & References (9)
Citations & References
Abstract
The TSC1 tumour suppressor hamartin regulates cell adhesion through ERM proteins and the GTPase Rho.
Journal:Nat Cell Biol
PubMed ID:10806479
'Loss of the tumour-suppressor gene TSC1 is responsible for hamartoma development in tuberous sclerosis complex (TSC), which renders several organs susceptible to benign tumours. Hamartin, the protein encoded by TSC1, contains a coiled-coil domain and is expressed in most adult tissues, although its function is unknown. Here we show that
CCR5 provides a signal for microbial induced production of IL-12 by CD8 alpha+ dendritic cells.
Journal:Nat Immunol
PubMed ID:10881180
'The activation of dendritic cells (DC) to produce interleukin 12 (IL-12) is thought to be a key step in the initiation of cell-mediated immunity to intracellular pathogens. Here we show that ligation of the C-C chemokine receptor (CCR) 5 can provide a major signal for the induction of IL-12 synthesis
Helicobacter pylori evolution during progression from chronic atrophic gastritis to gastric cancer and its impact on gastric stem cells.
Journal:Proc Natl Acad Sci U S A
PubMed ID:18332421
'We have characterized the adaptations of Helicobacter pylori to a rarely captured event in the evolution of its impact on host biology-the transition from chronic atrophic gastritis (ChAG) to gastric adenocarcinoma-and defined the impact of these adaptations on an intriguing but poorly characterized interaction between this bacterium and gastric epithelial
Versatile, high-resolution anterograde labeling of vagal efferent projections with dextran amines.
Journal:J Neurosci Methods
PubMed ID:19056424
'None of the anterograde tracers used to label and investigate vagal preganglionic neurons projecting to the viscera has proved optimal for routine and extensive labeling of autonomic terminal fields. To identify an alternative tracer protocol, the present experiment evaluated whether dextran conjugates, which have produced superior results in the CNS,
Müller glia are a potential source of neural regeneration in the postnatal chicken retina.
Journal:Nat Neurosci
PubMed ID:11224540
The retina of warm-blooded vertebrates is believed to be incapable of neural regeneration. Here we provide evidence that the retina of postnatal chickens has the potential to generate new neurons. In response to acute damage, numerous Müller glia re-entered the cell cycle, and shortly thereafter, expressed CASH-1, Pax6 and Chx10,