SYPRO™ Protein Gel Stains

SYPRO protein gel stains are sensitive, easy-to-use fluorescent stains for total proteins separated by polyacrylamide gel electrophoresis (PAGE). Stained proteins can be viewed with a standard UV or blue-light transilluminator, or imaging equipment containing the appropriate filters or laser. SYPRO protein gel stains offer many advantages over Coomassie Blue and silver staining, including a fast, one-step staining protocol requiring no destaining, a linear dynamic range over three orders of magnitude, and very little protein-to-protein variation in staining.

SYPRO Ruby Protein Gel Stain features include:

• Convenient—ready-to-use formulation
• High sensitivity—detection to 0.25 to 1 ng
• Effective staining for 1D and 2D gels
• Can be multiplexed with other gel stains such as Pro-Q Diamond phosphoprotein gel stain or Pro-Q Emerald 300 glycoprotein gel stain
• Compatible with subsequent analysis of proteins by Edman-based sequencing or mass spectrometry

SYPRO Orange and SYPRO Red protein gel stain features include:

• Sensitive—detection to 4 to 8 ng
• Fast—rapid staining time of 10–60 min
• Best for 1D gels
• SYPRO Orange: Ex: 300, 470 nm, Em: 570 nm
• SYPRO Red: Ex: 300, 550 nm, Em: 630 nm

SYPRO Tangerine Protein Gel Stain features include:

• Sensitive—detection to 4 to 8 ng
• Fast—rapid staining time of 10 to 60 minutes
• Best for 1D gels
• Does not require fixation with acetic acid
• Compatible with subsequent gel staining, western blotting, zymography, electroelution or mass spectrometry