pYES2 Yeast Expression Vector

Citations & References (35)

Invitrogen™

pYES2 Yeast Expression Vector

The pYES2 vector is designed for native expression of your protein of interest in S. cerevisiae. It contains the URA3Read more

Catalog Number	Quantity
V82520	20 μg

Catalog number V82520

Price (TWD)

Quantity:

20 μg

The pYES2 vector is designed for native expression of your protein of interest in S. cerevisiae. It contains the URA3 gene for selection in yeast and 2μ origin for high-copy maintenance.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Antibiotic Resistance BacterialAmpicillin (AmpR)

Product TypeYeast Expression Vector

Quantity20 μg

VectorpYES

Cloning MethodRestriction Enzyme/MCS

Product LineYES

PromoterGAL1

Protein TagUntagged

Unit Size20 µg

Contents & Storage

The pYES2 vector kit includes 20 μg of expression vector, a TOP10F´ E. coli stab, and an INVSc1 yeast stab. Store the vector at -20°C. Store the TOP10F´ and INVSc1 stabs at +4°C. All reagents are guaranteed stable for 6 months when stored properly.

Frequently asked questions (FAQs)

What are the different kinds of media used for culturing Pichia pastoris and S. cerevisiae?

Following are the rich and minimal media used for culturing Pichia pastoris and S. cerevisiae:

Rich Media:
S. cerevisiae and Pichia pastoris
YPD (YEPD): yeast extract, peptone, and dextrose
YPDS: yeast extract, peptone, dextrose, and sorbitol

Pichia pastoris only
BMGY: buffered glycerol-complex medium
BMMY: buffered methanol-complex medium

Minimal Media (also known as drop-out media):
S. cerevisiae
SC (SD): Synthetic complete (YNB, dextrose (or raffinose or galactose), and amino acids)

Pichia pastoris
MGY: minimal glycerol medium
MD: minimal dextrose
MM: minimal methanol
BMGH: buffered minimal glycerol
BMMH: buffered minimal methanol

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Will the Saccharomyces cerevisiae alpha-factor secretion signal be recognized by Schizosaccharomyces pombe?

S. pombe cannot generate P factor when P factor is replaced for alpha in the alpha factor gene. It can, however, produce alpha factor when alpha is replaced for P in the P factor gene. This is negative evidence that S. pombe can process its own mating factor cleavage sites, but not all the cleavage sites of the S. cerevisiae alpha factor. It is better to use a more generic signal sequence (rather than a pre- pro- signal sequence such as alpha). If it is necessary to go the pre- pro- route, it is better to use the S. pombe P factor leader rather than the S. cerevisiae alpha leader.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

For galactose induction of expression in S. cerevisiae, can I include additional carbon sources in the media to increase yeast growth without repressing expression from the GAL promoter?

Some researchers choose to grow yeast in medium containing 2% galactose as the sole carbon source during induction. However, yeast typically grow more quickly in induction medium containing 2% galactose plus 2% raffinose. Raffinose is a good carbon source for yeast, and unlike glucose, does not repress transcription from the GAL promoter. Raffinose is a trisaccharide of galactose, glucose, and fructose linked in that order. Most yeast can cleave the glucose-fructose bond, but not the galactose-glucose bond. Fructose is then used as a carbon source.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Which S. cerevisiae yeast strain do your kits contain?

We offer the INVSc1 yeast strain. It is a diploid strain for expression purposes only. It does not sporulate well and is therefore not suited for yeast genetic studies. The genotype and phenotype of the INVSc1 strain are as follows:

Genotype: MATa his3D1 leu2 trp1-289 ura3-52/MATalpha his3D1 leu2 trp1-289 ura3-52
Phenotype: His-, Leu-, Trp-, Ura-
Note that INVSc1 is auxotrophic for histidine, leucine, tryptophan, and uracil. The strain will not grow in SC minimal medium that is deficient in histidine, leucine, tryptophan, and uracil.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Can old premixed lithium acetate buffers be used for preparing and transforming Saccharomyces cerevisiae?

Stock buffers of TE, lithium acetate, and PEG can be stored. However, the combined solution used to prepare the cells for transformation must be made fresh every time. There is a loss in transformation efficiency if the solutions are not freshly prepared.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

View all pYES2 Yeast Expression Vector FAQs

Citations & References (35)

Citations & References

Abstract

The Arabidopsis thaliana proton transporters, AtNhx1 and Avp1, can function in cation detoxification in yeast.

Authors:Gaxiola RA, Rao R, Sherman A, Grisafi P, Alper SL, Fink GR

Journal:Proc Natl Acad Sci U S A

PubMed ID:9990049

Overexpression of the Arabidopsis thaliana vacuolar H+-pyrophosphatase (AVP1) confers salt tolerance to the salt-sensitive ena1 mutant of Saccharomyces cerevisiae. Suppression of salt sensitivity requires two ion transporters, the Gef1 Cl- channel and the Nhx1 Na+/H+ exchanger. These two proteins colocalize to the prevacuolar compartment of yeast and are thought to ... More

The DExH box protein Suv3p is a component of a yeast mitochondrial 3'- to-5' exoribonuclease that suppresses group I intron toxicity.

Authors:Margossian SP, Li H, Zassenhaus HP, Butow RA

Journal:Cell

PubMed ID:8565066

The yeast mitochondrial protein Suv3p is a putative NTP-dependent RNA helicase. Here we report that in cells lacking Suv3p, there is an approximately 50-fold increase in the excised form of the group I intron omega of the mitochondrial 31S rRNA gene. Surprisingly, little mature 21S rRNA accumulates in those cells; ... More

A mouse homolog of the Escherichia coli recA and Saccharomyces cerevisiae RAD51 genes.

Authors:Morita T, Yoshimura Y, Yamamoto A, Murata K, Mori M, Yamamoto H, Matsushiro A

Journal:Proc Natl Acad Sci U S A

PubMed ID:8341671

'Analysis of mitotic and meiotic recombination in mammalian cells has been hampered by the complexity of the reactions involved as well as lack of mutants. Furthermore, none of the genes involved in the process has yet been identified. In budding yeast, Saccharomyces cerevisiae, the RAD51 gene is essential along with ... More

SALSA, a variant of yeast SAGA, contains truncated Spt7, which correlates with activated transcription.

Authors: Sterner David E; Belotserkovskaya Rimma; Berger Shelley L;

Journal:Proc Natl Acad Sci U S A

PubMed ID:12186975

'Spt-Ada-Gcn5 acetyltransferase (SAGA) is a previously described histone acetyltransferase/transcriptional coactivator complex in yeast. At promoters of certain genes (HIS3 and TRP3), SAGA has an inhibitory function involving a nonproductive TATA-binding protein interaction mediated by the Spt3 and Spt8 subunits. Related to this, Spt8-less SAGA is a major form of the ... More

Proteins connecting the nuclear pore complex with the nuclear interior.

Authors:Strambio-de-Castillia C, Blobel G, Rout MP

Journal:J Cell Biol

PubMed ID:10085285

'While much has been learned in recent years about the movement of soluble transport factors across the nuclear pore complex (NPC), comparatively little is known about intranuclear trafficking. We isolated the previously identified Saccharomyces protein Mlp1p (myosin- like protein) by an assay designed to find nuclear envelope (NE) associated proteins ... More

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