Cellfectin® II Reagent is a cationic-lipid formulation designed for optimal transfection of insect cells. Cellfectin® II is an improved version of the original Cellfectin® Reagent. In addition to providing the same great performance as Cellfectin®, Cellfectin® II Reagent is manufactured to ensure lot-to-lot consistency, and is optimized to be used in a faster protocol. Transfection with Cellfectin® II Reagent leads to consistent and efficient transfection of Sf9, Sf21, and High Five™ cells when using BaculoDirect™ and InsectSelect™ Expression Systems. Cellfectin® II Reagent can also be used to transfect adherent or suspension mammalian cells in serum-containing or serum-free media.