Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
產品號碼 | 包裝規格 | 價格 (TWD) | 供應狀況 | 數量 | |
---|---|---|---|---|---|
11965084 | 1,000 mL | 價格 2,050.00 線上優惠 1,435.00 您的價格 | *** | ||
11965092 亦稱為 11965-092 | 500 mL | 價格 1,230.00 線上優惠 861.00 您的價格 | *** | ||
11965118 亦稱為 11965-118 | 10 x 500 mL | 價格 10,100.00 線上優惠 7,751.00 您的價格 | *** | ||
11965126 | 6 x 1,000 mL | 價格 9,800.00 線上優惠 6,981.00 您的價格 | *** | ||
11965167 亦稱為 11965-167 | 5 L | 價格 12,100.00 線上優惠 8,470.00 (01-Jul-2024 結束) 您的價格 | *** | ||
11965175 亦稱為 11965-175 | 10 L | 價格 19,400.00 線上優惠 13,580.00 (01-Jul-2024 結束) 您的價格 | *** |
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
With | Without |
• High Glucose | • Sodium Pyruvate |
• L-glutamine | • HEPES |
• Phenol Red |
The complete formulation is available.
Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.