引用資料與參考文獻 (3)

Invitrogen™

Gateway™ pcDNA™-DEST53 Vector

To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast,深入閱讀

產品號碼	Quantity
12288015	6 μg

產品號碼 12288015

價格 (TWD)

Quantity:

價格 (TWD)

To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway™ destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available.

Additional materials required, available separately: Gateway™ entry clone, Gateway™ LR Clonase™ enzyme mix, and reaction buffer.

For Research Use Only. Not for use in diagnostic procedures.

Constitutive or Inducible SystemConstitutive

Delivery TypeTransfection

For Use With (Application)Reporter Assays

Product TypeGateway System Destination Expression Vector

Quantity6 μg

Reporter GeneGFP (Cycle 3)

Selection Agent (Eukaryotic)Geneticin™ (G-418)

VectorpDEST, pcDNA

Cloning MethodGateway

Product LineGateway, pcDNA

PromoterCMV

Protein TagGFP (Cycle 3)

Unit SizeEach

內容物與存放

All destination vectors are provided lyophilized and supercoiled.

常見問答集 (常見問題)

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all Gateway™ pcDNA™-DEST53 Vector FAQs

引用資料與參考文獻 (3)

引用資料與參考文獻

Abstract

Interaction codes within the family of mammalian Phox and Bem1p domain-containing proteins.

Authors:Lamark T, Perander M, Outzen H, Kristiansen K, Ã˜vervatn A, Michaelsen E, BjÃ¸rkÃ¸y G, Johansen T,

Journal:J Biol Chem

PubMed ID:12813044

'The Phox and Bem1p (PB1) domain constitutes a recently recognized protein-protein interaction domain found in the atypical protein kinase C (aPKC) isoenzymes, lambda/iota- and zeta PKC; members of mitogen-activated protein kinase (MAPK) modules like MEK5, MEKK2, and MEKK3; and in several scaffold proteins involved in cellular signaling. Among the last ... More

MARK4 is a novel microtubule-associated proteins/microtubule affinity-regulating kinase that binds to the cellular microtubule network and to centrosomes.

Authors:Trinczek B, Brajenovic M, Ebneth A, Drewes G,

Journal:J Biol Chem

PubMed ID:14594945

The MARK protein kinases were originally identified by their ability to phosphorylate a serine motif in the microtubule-binding domain of tau that is critical for microtubule binding. Here, we report the cloning and expression of a novel human paralog, MARK4, which shares 75% overall homology with MARK1-3 and is predominantly ... More

Mutation of melanosome protein RAB38 in chocolate mice.

Authors: Loftus Stacie K; Larson Denise M; Baxter Laura L; Antonellis Anthony; Chen Yidong; Wu Xufeng; Jiang Yuan; Bittner Michael; Hammer John A 3rd; Pavan William J;

Journal:Proc Natl Acad Sci U S A

PubMed ID:11917121

Mutations of genes needed for melanocyte function can result in oculocutaneous albinism. Examination of similarities in human gene expression patterns by using microarray analysis reveals that RAB38, a small GTP binding protein, demonstrates a similar expression profile to melanocytic genes. Comparative genomic analysis localizes human RAB38 to the mouse chocolate ... More