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SuperScript™ IV One-Step RT-PCR System
SuperScript™ IV One-Step RT-PCR System
Invitrogen™

SuperScript™ IV One-Step RT-PCR System

The Invitrogen SuperScript One-Step RT-PCR System, available with or without ezDNase, combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance.
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產品號碼IncludesNo. of Reactions
12594100Kit only100 Reactions
12595100Kit with ezDNase100 Reactions
產品號碼 12594100
價格 (TWD)
35,775.00
線上優惠
Ends: 31-Mar-2026
47,700.00
您節省 11,925.00 (25%)
Each
新增至購物車
Includes:
Kit only
No. of Reactions:
100 Reactions
Request bulk or custom format
價格 (TWD)
35,775.00
線上優惠
Ends: 31-Mar-2026
47,700.00
您節省 11,925.00 (25%)
Each
新增至購物車
The Invitrogen SuperScript One-Step RT-PCR System, available with or without ezDNase, combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance. With this convenient one-step formulation plus gene-specific primers, you can perform both cDNA synthesis from total RNA or mRNA and PCR amplification in a single tube.

Features of the SuperScript IV One-Step RT-PCR System include:

  • Superior performance with sensitivity down to 0.01 pg RNA, target length up to 13.8 kb, and the fastest one-step RT-PCR protocol
  • Reliable target detection even with suboptimally pure RNA samples
  • Two-phase hot-start activation mechanism for high sensitivity, specificity, and room temperature setup
  • Fast and easy gDNA removal for superior accuracy and confidence in results (SuperScript IV One-Step RT-PCR System with ezDNase only)

SuperScript IV RT is a proprietary MMLV RT mutant with increased processivity, thermostability, and inhibitor resistance, as well as reduced RNase H activity, for sensitive and efficient full-length cDNA synthesis. Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR. The combination of these two engineered enzymes produces enhanced RT-PCR yields and fidelity, as well as amplification of longer templates compared to other one-step RT-PCR kits.

The inclusion of ezDNase (SuperScript IV One-Step RT-PCR System with ezDNase only) for use upstream of the RT step further enhances the one-step RT-PCR workflow through an extremely simplified genomic DNA removal step that ensures the highest accuracy and confidence in one-step RT-PCR results.

For Research Use Only. Not for use in diagnostic procedures.
規格
Final Product TypePCR Amplified cDNA
FormatKit
Hot StartBuilt-In Hot Start
IncludesKit only
No. of Reactions100 Reactions
Optimal Reaction Temperature45°C to 60°C
PolymerasePlatinum SuperFi
Quantity1 Ea.
Reaction FormatMaster Mix
Reagent TypeReverse Transcription
Reverse TranscriptaseSuperScript IV
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
Size (Final Product)Up to 14 kb
Starting MaterialRNA
Technique1-step RT-PCR
Detection MethodGel Electrophoresis
GC-Rich PCR PerformanceHigh
PCR Method1-step RT-PCR
Reaction Speed10 min.
Unit SizeEach
內容物與存放

• SuperScript IV RT Mix (50 μL)
• 2X Platinum SuperFi RT-PCR Master Mix (2 x 1.25 mL)
• Nuclease-free water (2 x 1.25 mL)

Store at –20°C.

常見問答集 (常見問題)

In your SuperScript IV RT protocols, there is no ezDNase inactivation step. Will ezDNase be active to affect RNA or the RT reaction?

The Invitrogen ezDNase Enzyme is a novel DNase that is highly specific for double-stranded DNA. It has no activity on single-stranded DNA in RT reactions (primers or probes), or on RNA. The enzyme is also thermolabile—it is inactivated quickly at temperatures typical for the SuperScript IV RT reaction (e.g., 50 degrees C). The additional inactivation step is therefore not required for most RT-qPCR applications. If the RNA sample is to be used for RT-PCR of fragments ≥3 kb, incubate the sample for 5 minutes at 55 degrees C in the presence of 10 mM DTT to inactivate the enzyme.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Which SuperScript IV RT format do you recommend for real-time PCR applications?

For RT-qPCR applications we recommend using the Invitrogen SuperScript IV VILO Master Mix. The cDNA synthesis reaction setup with this master mix requires fewer pipetting steps and therefore reduces variation in the data. SuperScript IV RT, as a component of the master mix, offers the highest efficiency of cDNA synthesis step compared to competitors’ products.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Are there any significant changes in the SuperScript IV RT protocol compared to the SuperScript III RT protocol?

The only difference is that the incubation time for the reverse transcription reaction has been reduced to 10 minutes and inactivation time has been reduced to 5 minutes at 85 degrees C.

Can I get comparable cDNA yield and length using the SuperScript IV RT 10-minute protocol as when using the 50-minute protocol for SuperScript III RT?

When compared with SuperScript III RT (and other manufacturers’ RTs) in a synthesis reaction for a 9 kb cDNA, SuperScript IV RT performed successful synthesis in just 10 minutes and did so with comparable (or improved) yield (as shown by gel band density).

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Do you offer a one-step RT-qPCR kit containing SuperScript IV RT?

SuperScript IV RT is not recommended for one-step RT-qPCR applications. However, it is included in end-point one-step RT-PCR kits: https://thermofisher.com/ssiv-onestep

For one-step RT-qPCR, we recommend SuperScript III Flash Reverse Transcriptase or TaqPath RT-qPCR Master Mix products.

View all SuperScript™ IV One-Step RT-PCR System FAQs