Invitrogen Platinum II Green PCR Buffer is an innovative buffer optimized for use with Platinum II Taq Hot-Start DNA Polymerase. Due to its unique composition, the annealing temperature is 60°C for most primer pairs designed following the general design rules. Isostabilizing molecules in the buffer increase primer–template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize annealing temperature for each primer pair.

Platinum II Green PCR Buffer provides 1.5 mM MgCl₂ in the final reaction and is supplemented with a density reagent and two tracking dyes for direct loading of PCR products on gels.

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