McCoy's 5A (Modified) Medium
McCoy's 5A (Modified) Medium
McCoy's 5A (Modified) Medium
McCoy's 5A (Modified) Medium
引用資料與參考文獻 (3)
Gibco™

McCoy's 5A (Modified) Medium

McCoy's 5A (modified) Medium is a general purpose medium that supports the propagation of many types of primary cells, established深入閱讀
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產品號碼Quantity
16600082500 mL
1660010810 x 500 mL
產品號碼 16600082
價格 (TWD)
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Quantity:
500 mL
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McCoy's 5A (modified) Medium is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.


This McCoy's 5A is modified as follows:
WithWithout
• High Glucose• Sodium Pyruvate
• L-glutamine• HEPES
• Bacto-peptone 
• Phenol Red 


The complete formulation is available.

Using McCoy's 5A (modified) Medium
Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. This product also includes Dr. Hsu's addition of Hanks' salts to enable use outside a CO2 incubator. McCoy's 5A (modified) Medium requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS). McCoy's 5A (modified) Medium uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

規格
Cell LineRat fibroblasts
Cell TypeBiopsy Tissues
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco
Product TypeMcCoy's 5A Modified Medium
Quantity500 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin
FormLiquid
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesHigh Glucose, Glutamine, Phenol Red, Sodium Bicarbonate
Without AdditivesNo HEPES, No Sodium Pyruvate
Unit SizeEach
內容物與存放
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

常見問答集 (常見問題)

What is the shelf life of the DMEM, high glucose, pyruvate medium once the bottle is opened and the medium is supplemented?

We do not provide stability data for the product once it is opened as it would depend on the usage and storage conditions.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is it necessary to store DMEM, high glucose, pyruvate medium in the dark?

Yes, the medium should be stored in the dark because there are some components in the medium such as HEPES, Tryptophan, and Riboflavin, etc. that are sensitive to light.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all McCoy's 5A (Modified) Medium, 500 mL FAQs

引用資料與參考文獻 (3)

引用資料與參考文獻
Abstract
Novel kelch-like protein, KLEIP, is involved in actin assembly at cell-cell contact sites of Madin-Darby canine kidney cells.
Authors:Hara T, Ishida H, Raziuddin R, Dorkhom S, Kamijo K, Miki T,
Journal:Mol Biol Cell
PubMed ID:14668487
Dynamic rearrangements of cell-cell adhesion underlie a diverse range of physiological processes, but their precise molecular mechanisms are still obscure. Thus, identification of novel players that are involved in cell-cell adhesion would be important. We isolated a human kelch-related protein, Kelch-like ECT2 interacting protein (KLEIP), which contains the broad-complex, tramtrack, ... More
DMH1, a novel BMP small molecule inhibitor, increases cardiomyocyte progenitors and promotes cardiac differentiation in mouse embryonic stem cells.
Authors:Ao A, Hao J, Hopkins CR, Hong CC
Journal:PLoS One
PubMed ID:22848549
The possibility of using cell-based therapeutics to treat cardiac failure has generated significant interest since the initial introduction of stem cell-based technologies. However, the methods to quickly and robustly direct stem cell differentiation towards cardiac cell types have been limited by a reliance on recombinant growth factors to provide necessary ... More
A 2-D liquid separations/mass mapping method for interlysate comparison of ovarian cancers.
Authors: Kachman Maureen T; Wang Haixing; Schwartz Donald R; Cho Kathleen R; Lubman David M;
Journal:Anal Chem
PubMed ID:11985308
A two-dimensional liquid phase separation of proteins from whole cell lysates coupled on-line to an electrospray-ionization time-of-flight (ESI-TOF) mass spectrometer (MS) is used to map the protein content of ovarian surface epithelial cells (OSE) and an ovarian carcinoma-derived cell line (ES2). The two dimensions involve the use of liquid isoelectric ... More