L-Glutamine is an amino acid required for cell culture. L-glutamine participates in the formation of purine and pyrimidine nucleotides, amino sugars, glutathione, L-glutamate, other amino acids, and is used in protein synthesis and glucose production. L-glutamine, unlike most other amino acids, is not stable in solution. The rate at which this degradation proceeds is a function of time, temperature, and pH.

Using L-glutamine
L-glutamine (nonsterile) powder should be made as a filter-sterilized stock solution of 200 mM (or similar) in distilled water. The optimal concentration to use is dependent upon the cell type and medium used to culture the cells, but generally falls in the range of 2–6 mM.

Alternative product
We also offerGlutaMAX™ supplement as a stable alternative to L-glutamine.