CaptureSelect™ EPO Affinity Matrix

CaptureSelect EPO Affinity Matrix is designed for the purification of Erythropoietin (EPO) and/or Darbepoetin (DPO) from recombinant sources.

Features of the CaptureSelect EPO Affinity Matrix include:

• EPO purification with high purity and yield from recombinant sources
• The CaptureSelect EPO affinity ligand recognizes a proteinaceous epitope thereby covering all EPO glycosylation isoforms present in the feed
• Binding both EPO & DPO enabling purification of these molecules
• Efficient elution at neutral pH (50 mM Tris 0.5 M CaCl2 pH 7.0) and acidic pH (20 mM Citric acid pH 3.0)
• Excellent scalability
• Non-animal-derived

The high selectivity and yields obtained using CaptureSelect EPO Affinity Matrix enable a robust, fast, and efficient purification process with excellent purity in one step, eliminating the need for multiple purification stages.

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (V_HH) created using a proprietary technology. The V_HH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics

• Matrix: agarose-based
• Average particle size: 65μm ± 10μm
• Ligand: CaptureSelect EPO affinity ligand
• Ligand coupling method: epoxide coupling
• Binding capacity: > 6 mg EPO/mL resin
• Binding conditions: PBS or TBS (Tris buffered saline) pH 7.4
• Elution conditions: 50 mM Tris 0.5 M CaCl2 pH 7.0 (neutral), 20 mM citric acid, pH 3 (acidic)
• Flow characteristics: 50–200 cm/h (up to 2 bar)
• Formulation buffer: 20% (v/v) ethanol