Gibco™

Keratinocyte-SFM Supplement: Human Recombinant Epidermal Growth Factor (EGF 1-53) & Bovine Pituitary Extract (BPE)

Human recombinant Epidermal Growth Factor (EGF 1-53) and Bovine Pituitary Extract (BPE).深入閱讀

產品號碼	Quantity
37000015	1 mL

產品號碼 37000015

價格 (TWD)

Quantity:

Human recombinant Epidermal Growth Factor (EGF 1-53) and Bovine Pituitary Extract (BPE).

For Research Use Only. Not for use in diagnostic procedures.

Cell TypeKeratinocyte Cells, Epithelial Cells

Culture TypeMammalian Cell Culture

Quantity1 mL

Serum LevelSerum-Free

Shelf Life18 Months

Shipping ConditionDry Ice

SpeciesHuman

ClassificationSerum-free

Product TypeKeratinocyte-SFM Supplement

SterilitySterile

Sterilization MethodSterile-filtered

With AdditivesPhenol Red

Unit SizeEach

內容物與存放

Store in freezer (-5 to -30°C).

常見問答集 (常見問題)

Are the Keratinocyte SFM basal medium and supplement kit available separately?

The basal medium is not available for purchase individually, and can only be ordered as part of the Keratinocyte SFM kit (catalog # 17005-042). The supplements are available separately: Bovine Pituitary Extract is catalog # 13028-014 and the EGF is catalog # PHG0311.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which serum-free media can be used to culture mouse keratinocytes?

The Keratinocyte SFM without calcium chloride (Cat. No. 37010-022) is appropriate for the culture of mouse keratinocytes. The medium should be supplemented with calcium chloride at a final concentration of 0.02 mM.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which serum-free media can be used to culture human keratinocytes?

There are a variety of options for serum-free culture of human keratinocytes. Keratinocyte SFM (Cat. No. 17005-042) is a widely used kit that includes the basal medium, bovine pituitary extract (BPE) and Epidermal Growth Factor (EGF). If you would like a serum-free, defined culture system, the options are the Defined Keratinocyte SFM kit (Cat. No. 10744-019) or Epilife medium (Cat. No. M-EPI-500-CA) supplemented with EDGS (Cat. No. S-012-5). If you would like an animal origin free defined system, Epilife medium (Cat. No. M-EPI-500-CA) with S7 supplement (Cat. No. S-017-5) is an excellent choice.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

For how long can I store Keratinocyte-SFM at 4 degrees C?

The base Keratinocyte-SFM has a shelf life of 12 months when stored at 2 to 8 degrees C, protected from light. Keratinocyte-SFM Supplement has a shelf life of 18 months when stored at -20 to 5 degrees C. The complete media should not be stored for longer than 4 weeks at 4 degrees C

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.