Blocker™ BLOTTO in TBS
Blocker™ BLOTTO in TBS
Thermo Scientific™

Blocker™ BLOTTO in TBS

Blocker BLOTTO Blocking Buffer is a ready-to-use 5% solution of nonfat powdered milk in Tris-buffered saline for western blot, ELISA,深入閱讀
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產品號碼Quantity
375301 L
產品號碼 37530
價格 (TWD)
5,600.00
Online offer
Ends: 31-Dec-2025
7,990.00
您節省 2,390.00 (30%)
Each
新增至購物車
Quantity:
1 L
Request bulk or custom format
價格 (TWD)
5,600.00
Online offer
Ends: 31-Dec-2025
7,990.00
您節省 2,390.00 (30%)
Each
新增至購物車
Blocker BLOTTO Blocking Buffer is a ready-to-use 5% solution of nonfat powdered milk in Tris-buffered saline for western blot, ELISA, IHC, and other detection methods.

Compare and view all available blocking buffers

Features of Blocker BLOTTO Blocking Buffer:
• Format—ready-to-use 1X TBS solution; can be diluted as needed
• Formulation—formulated with anti-foaming agent and thimerosal-free preservative

Not recommended for avidin-based techniques because it contains some endogenous biotin.

For Research Use Only. Not for use in diagnostic procedures.
規格
Chemical Name or MaterialBlocker BLOTTO
Recommended StorageStore at 4°C.
Concentration1X
For Use With (Application)Western Blot
Physical FormLiquid
Product LineBlocker
Quantity1 L
Unit SizeEach

常見問答集 (常見問題)

How can I reduce background bands in my Western blot?

Optimize the concentration of primary and secondary antibodies. In some cases, increasing the concentration of blocking agent (BSA or non-fat dry milk) or usiing an alternative blocking solution such as Starting Block or SuperBlock may reduce background signal. After incubation with the primary antibody, wash at least 2 times with TBST (include 0.5 M NaCl in one or more of the wash steps). Avoid Nonidet P40 or Triton X-100 in buffers because protein detection is decreased when these detergents are used.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.