TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG
TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG
Actual product may vary
引用資料與參考文獻 (43)
Applied Biosystems™

TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG

TaqMan Fast Universal Master Mix (2X), No AmpErase UNG, delivers results in 40 minutes for 40 cycles of PCR in深入閱讀
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產品號碼No. of Reactions
4352042250 Reactions
4366072500 Reactions
43678465000 Reactions
43660731250 Reactions
43641032500 Reactions
產品號碼 4352042
價格 (TWD)
10,800.00
Offre exceptionnelle en ligne
Ends: 31-Dec-2025
13,400.00
您節省 2,600.00 (19%)
Each
新增至購物車
No. of Reactions:
250 Reactions
價格 (TWD)
10,800.00
Offre exceptionnelle en ligne
Ends: 31-Dec-2025
13,400.00
您節省 2,600.00 (19%)
Each
新增至購物車
TaqMan Fast Universal Master Mix (2X), No AmpErase UNG, delivers results in 40 minutes for 40 cycles of PCR in a 20-μL reaction volume using the Applied Biosystems 7900HT and Fast 7500 Fast real-time PCR systems. The optimized formulation contains AmpliTaq Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and allowing room temperature reaction setup. Additionally, a proprietary ROX dye serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations, for superb precision on Applied Biosystems real-time PCR instruments.

Convenience
Everything you need for TaqMan-based qPCR amplification and detection in a single-tube format. TaqMan Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR.

Compatibility
TaqMan Fast Universal PCR Master Mix (2X), No AmpErase UNG, is compatible with TaqMan Gene Expression assays.

Alternative product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Fast Universal PCR Master Mix and added additional capabilities for your gene expression analysis.

For Research Use Only. Not for use in diagnostic procedures.
規格
Fidelity (vs. Taq)2 X
For Use With (Equipment)7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
FormatTube
No. of Reactions250 Reactions
Passive Reference DyeROX (Pre-mixed)
PolymeraseAmpliTaq DNA Polymerase
Product LineAmpErase, TaqMan
Product TypeFast Universal PCR Master Mix
Quantity250 reactions
Sample TypeDNA (Genomic), cDNA
Shipping ConditionWet Ice
Sufficient For250 Reactions
Volume20 μL
Concentration2X
Detection MethodPrimer-probe
For Use With (Application)Gene Expression, Genotyping, miRNA analysis
GC-Rich PCR PerformanceHigh
PCR MethodqPCR
Reaction SpeedFast
Unit SizeEach
內容物與存放
TaqMan™ Fast Universal PCR Master Mix, No AmpErase™ UNG Kit contains two tubes of 1.25 mL each sufficient for 250 20-μL PCR reactions. Store at 2-8°C

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

常見問答集 (常見問題)

Can SNP assays be run using the TaqMan Fast Universal PCR Master Mix (Cat. No. 4352042)?

It may be technically feasible, but we have not fully validated TaqMan SNP Genotyping assays on the Applied Biosystems 7500 Fast System and therefore cannot recommend it.

What real-time PCR applications can I perform using the TaqMan Fast Universal PCR Master Mix (Cat. No. 4352042)?

You can perform experiments for Absolute Quantitation and Relative Quantitation with fast cycling. However, Allelic Discrimination (SNP) assays and plus/minus assays using internal positive controls cannot be run with fast reagents and protocols.

引用資料與參考文獻 (43)

引用資料與參考文獻
Abstract
Genetic analysis of hepatitis C virus with defective genome and its infectivity in vitro.
Authors:Sugiyama K, Suzuki K, Nakazawa T, Funami K, Hishiki T, Ogawa K, Saito S, Shimotohno KW, Suzuki T, Shimizu Y, Tobita R, Hijikata M, Takaku H, Shimotohno K
Journal:J Virol
PubMed ID:19369330
Replication and infectivity of hepatitis C virus (HCV) with a defective genome is ambiguous. We molecularly cloned 38 HCV isolates with defective genomes from 18 patient sera. The structural regions were widely deleted, with the 5' untranslated, core, and NS3-NS5B regions preserved. All of the deletions were in frame, ... More
Down Regulation of Genes Involved in T Cell Polarity and Motility during the Induction of Heart Allograft Tolerance by Allochimeric MHC I
Authors:Lisik, W; Tejpal, N; Gong, YQ; Skelton, TS; Ganachari, M; Bremer, EG; Kloc, M; Ghobrial, RM
Journal:
PubMed ID:
'Background: The allochimeric MHC class I molecule [α1h1/u]-RT1. Aa that contains donor-type (Wistar Furth, WF; RT1u) epitopes displayed on recipient-type (ACI, RT1a) administered in conjunction with sub-therapeutic dose of cyclosporine (CsA) induces indefinite survival of heterotopic cardiac allografts in rat model. In vascularized transplantation models, the spleen contributes to graft ... More
Activation of Macrophages by Polysaccharide-protein Complex from Lycium barbarum L.
Authors:Chen, ZS; Soo, MY; Srinivasan, N; Tan, BKH; Chan, SH
Journal:
PubMed ID:
'Macrophages play crucial roles in innate immunity. This paper reports that a polysaccharide-protein complex isolated from Lycium barbarum (LBP) is able to activate macrophages. LBP was isolated from Lycium barbarum fruit and separated to five homogenous fractions, designated LBPF1, LBPF2, LBPF3, LBPF4 and LBPF5. It was found that LBP (50 ... More
Fusion of Epstein-Barr virus nuclear antigen-1-derived glycine-alanine repeat to trans-dominant HIV-1 Gag increases inhibitory activities and survival of transduced cells in vivo
Authors:Hammer, D; Wild, J; Ludwig, C; Asbach, B; Notka, F; Wagner, R
Journal:
PubMed ID:
'Trans-dominant human immunodeficiency virus type 1 (HIV-1) Gag derivatives have been shown to efficiently inhibit late steps of HIV-1 replication in vitro by interfering with Gag precursor assembly, thus ranking among the interesting candidates for gene therapy approaches. However, efficient antiviral activities of corresponding transgenes are likely to be counteracted ... More
Prevalence of Chlamydophila psittaci in wild birds-potential risk for domestic poultry, pet birds, and public health?
Authors:Zweifel, D; Hoop, R; Sachse, K; Pospischil, A; Borel, N
Journal:
PubMed ID:
'To determine the prevalence of Chlamydophila psittaci in wild birds, cloacal swabs from 527 songbirds, 442 waterfowl, 84 feral pigeons, and 38 cormorants were examined by Chlamydiaceae-specific real-time polymerase chain reaction (PCR) and ArrayTube microarray assays for chlamydial species determination and genotyping of C. psittaci. Inconclusive cases were further characterized ... More
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