This easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™/SYBR™ Green I, VIC™/JOE™, and ROX™ dyes on the StepOne™ Real-Time PCR System.

Features of the StepOne™ Real-Time PCR System Spectral Calibration Kit include:

• 48-well optical reaction plates preloaded with dyes (FAM™/SYBR™ Green I, VIC™/JOE™, and ROX™) are provided for quick and easy calibration of the StepOne™ Real-Time PCR System
• Easy-to-use and provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture
• Includes the Passive Reference I dye standard for signal normalization in all real-time and endpoint reactions

The Applied Biosystems™ StepOne™ Real-Time PCR System Spectral Calibration Kit contains one background plate and three preloaded, 48-well fast optical reaction plates containing five dye standards.