TaqMan™ Sample-to-SNP™ Kit (5 mL sample prep, 1 mL PCR)
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Applied Biosystems™

TaqMan™ Sample-to-SNP™ Kit (5 mL sample prep, 1 mL PCR)

The Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit allows you to go from Biological Sample to Genotyping Results in less than one深入閱讀
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產品號碼Quantity
44033131 kit
產品號碼 4403313
價格 (TWD)
10,200.00
Online offer
Ends: 31-Dec-2025
12,700.00
您節省 2,500.00 (20%)
Each
新增至購物車
Quantity:
1 kit
價格 (TWD)
10,200.00
Online offer
Ends: 31-Dec-2025
12,700.00
您節省 2,500.00 (20%)
Each
新增至購物車
The Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit allows you to go from Biological Sample to Genotyping Results in less than one hour. The TaqMan™ Sample-to-SNP™ Kit combines DNA extraction and TaqMan™ chemistry-based genotyping in a streamlined protocol to provide results from any sample in less than one hour.

• DNA Extract All Reagents kits provide PCR-ready DNA from a wide variety of sample types ranging from blood to buccal swabs to plant tissues in 5 min
• The TaqMan™™ GTXpress™ Master Mix provides robust PCR amplification of extracted DNA in less than 50 min
• Compatible with both Fast and Standard mode thermal cycling conditions
• Pre-mixed components stored at 2°–8°C significantly reduce assay setup time
• Validated with all types of Applied Biosystems™ TaqMan™ Genotyping Assays
• Extended PCR cycling for poorly amplified or sample-limited reactions

The kit includes reagents sufficient for 100 sample preparation reactions at 50 μL/ reaction and 400 PCR reactions at 5 μL for each reaction.

Please note:
The DNA Extract All reagents are not available for individual sale. They are only available as a part of the TaqMan™ Sample-to-SNP™ Kit.
For Research Use Only. Not for use in diagnostic procedures.
規格
For Use With (Equipment)7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, GeneAmp 9700, StepOne™, Fast Mode, StepOne™, Standard Mode, Veriti Thermal Cycler, StepOne™ Standard Mode, StepOne™ Fast Mode
Genotyping TargetSNPs (Unknown or Numerous), SNPs (Known)
Hot StartBuilt-In Hot Start
Passive Reference DyeROX (Pre-mixed)
PolymeraseAmpliTaq DNA Polymerase
Product LineSample-to-SNP, TaqMan
Product TypeSample to SNP Kit
Purity or Quality GradeUP (Ultra Pure)
Quantity1 kit
Sample TypeBuccal Sample, Cell Cultures, Hair with Follicle, Leaf Punch or Needle, Seed Chip, Tail Biopsy (Mouse), Tail Biopsy (Rat), Tissue (FFPE), Whole Blood
Sufficient For100 Reactions
TechniqueSNP Genotyping (Real-Time PCR-Based)
Volume5 mL
Detection MethodPrimer-probe
GC-Rich PCR PerformanceLow
PCR MethodqPCR
Reaction SpeedFast or Standard
Unit SizeEach
內容物與存放
Lysis Reagents (2° to 8°C): 5 mL each Lysis and DNA Stabilization Solution; Master Mix (15° to 25°C ): 1 mL 2X master mix with AmpliTaq™ Fast DNA Polymerase, UP, dNTPs, Tracking Dye and ROX™ Dye.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

常見問答集 (常見問題)

When designing probes to perform Allelic Discrimination (SNP assays), are there any alternatives to using probes longer than 30bp in order to get the appropriate melting temperature?

Applied Biosystems TaqMan MGB (minor groove binder) probes would be appropriate for Allelic Discrimination/SNP Genotyping probe designs. TaqMan MGB probes have a minor groove binder at the end of the probe. This minor groove binder increases the Tm of probes, allowing the use of shorter probes. Consequently, the TaqMan MGB probes exhibit greater differences in Tm values between matched and mismatched probes, which provides more accurate allele discrimination.

For information on the design of TaqMan MGB probes for Allelic Discrimination/SNP assays using Primer Express software, please refer to our guide "Designing TaqMan MGB Probe and Primer Sets for Allelic Discrimination Assays Using Primer Express".