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Invitrogen™

Jump-In™ Fast Gateway™ System

The Jump-In™ Fast Gateway™ System is intended for rapid engineering of mammalian cell lines through the stable and irreversible integration of your gene of interest (GOI) at specific genomic loci (pseudo attP sites).
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產品號碼Quantity
A1089320 Reactions
產品號碼 A10893
價格 (TWD)
-
Quantity:
20 Reactions

The Jump-In™ Fast Gateway™ System is intended for rapid engineering of mammalian cell lines through the stable and irreversible integration of your gene of interest (GOI) at specific genomic loci (pseudo attP sites). Following stable integration, only 5-10 clones need to be analyzed to identify clones with high expression levels.

The Jump-In™ Fast Gateway™ System consists of:

  • The Jump-In™ Fast Gateway™ Core Kit containing the promoter-less pJTI™ Fast DEST expression vector and a vector for transient expression of the PhiC31 integrase.
  • The MultiSite Gateway™ Pro Plus kit for cloning of up to 4 gene fragments into the promoter-less pJTI™ Fast DEST expression vector.

For Research Use Only. Not for use in diagnostic procedures.

規格
Cloning MethodGateway™
Constitutive or Inducible SystemConstitutive
Delivery TypeTransfection
Expression SystemMammalian
For Use With (Application)Protein Expression
High-throughput CompatibilityHigh-throughput Compatible
Key FunctionsStable Cell Line Development, Targeted Integration, Clone your own Promoter
No. of Reactions20 reactions
Product LineJump-In
Product TypeCloning Kit
Protein TagUntagged
Quantity20 Reactions
Selection Agent (Eukaryotic)Hygromycin
Selection Marker EukaryoticHygroR
Cell TypeMammalian
PromoterNone (Promoterless)
VectorJump-In Vectors, pJTI
Unit SizeEach
內容物與存放
Jump-In™ Fast Gateway™ System is composed of the Jump-In™ Fast Gateway™ Core Kit and the MultiSite Gateway™ Pro Plus Kit (includes One Shot™ Mach1™ T1R Chemically Competent Cells, and LR Clonase™ II Plus and BP Clonase™ II enzymes).

This kit has components that can also be sold separately.

Store cells at -80°C.
Store Clonase™ enzymes at -20°C for up to 6 months, or at -80°C for long-term storage.
Store all other components, including vectors, at -20°C.

常見問答集 (常見問題)

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all Jump-In™ Fast Gateway™ System FAQs