TrueMark™ MSI Assay

The Applied Biosystems TrueMark MSI Assay is a low-input, single-amplification solution for the rapid identification of microsatellite instability (MSI) in formalin-fixed paraffin-embedded (FFPE) samples from multiple tumor tissue types for research applications. The TrueMark MSI Assay leverages an expanded 6-dye set to incorporate additional markers. The TrueMark MSI Assay is best suited for the analysis of colon cancer samples for research. After careful verification with your samples and your markers of interest, the free auto-calling software may be used without a tumor-normal match for colon cancer samples. The assay is compatible with the Applied Biosystems SeqStudio, SeqStudio Flex, and 3500 Series genetic analyzers.

MSI analysis software
The associated TrueMark MSI Analysis Software (included at no additional charge) is designed specifically for the TrueMark MSI Assay to enable automated calling of markers and samples, offering greater ease of analysis by reducing the manual QC, analysis, and review required to determine sample status. The automated calling is best used after a verification study and can be used with tumor-normal matched samples as well as without tumor-normal pairs.

Download TrueMark MSI Analysis Software ›

The TrueMark MSI Assay features include:
• Expanded coverage—13 MSI markers
    --BAT-25, BAT-26, BAT-40, CAT-25, NR-21, NR-22, NR-24, NR-27 (publicly known MSI markers)
    --ABI-16, ABI-17, ABI-19, ABI-20A, ABI-20B (MSI markers proprietary to Thermo Fisher Scientific)
    --PentaD, TH-01 (sample ID markers)
• Optional normal control—no requirement for use of tumor-normal match for interpretation
• Low input—use as little as 2 ng FFPE-derived DNA
• Automated calling—analysis software includes automated calling of markers and samples to accelerate initial data interpretation
• Simple analysis software—streamline QC, analysis, and review of samples

Analysis software for download
• TrueMark MSI Analysis Software

Supplied reagents
• TrueMark MSI Primer mix
• TrueMark MSI Master mix
• TrueMark MSI No Template Control
• TrueMark MSI Amplification Control

Required reagents not supplied in the TrueMark MSI Assay
• GeneScan 600 LIZ Size Standard v2.0
• Hi-Di Formamide
• DS-36 Matrix Standard (Dye Set J6)

The TrueMark MSI Assay has been tested with RecoverAll Total Nucleic Acid Isolation Kit for FFPE and Qiagen QIAamp™ DSP DNA FFPE Tissue Kit for sample preparation. Please note these are not included in the kit.