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引用資料與參考文獻 (4)
Thermo Scientific™
EasyPep™ MS Sample Prep Kits
Generate high quality proteomic samples for MS analysis in less than four hours using optimized EasyPep MS Sample Prep kits.
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變更視圖
| 產品號碼 | Description |
|---|---|
| A45733 | MS Sample Prep Kit |
| A40006 | Mini MS Sample Prep Kit |
| A57864 | Micro MS Sample Prep Kit |
| A45734 | Maxi Sample Prep Kit |
| A45735 | Lysis Buffer |
| A57865 | Peptide Clean-up Plate |
產品號碼 A45733
價格 (TWD)
-
Description:
MS Sample Prep Kit
EasyPep Mini MS Sample Prep Kits enable efficient and reproducible processing of cultured mammalian cells and tissues for proteomic MS analysis. The kit contains pre-formulated buffers, MS-grade enzyme mix, peptide clean-up columns, and an optimized protocol to generate MS-compatible peptide samples in less than three hours. Kits are optimized to process protein samples from 10–100 μg with a high yield of MS-ready peptides.
• Complete—includes pre-formulated reagents for lysis through digestion, peptide clean-up columns, and an optimized protocol for up to 20 samples
• Optimized—streamlined protocol and reagents minimize the number of steps and time time needed to process samples
• Flexible—reagents and protocol have been verified using cells, plasma, and tissue samples for 10 to 100 μg samples
• Time-saving—sample processing time reduced from more than one day to less than three hours
• Compatible—final preparation is ready for direct MS analysis and other downstream applications, including TMT labeling
The sample preparation of peptides for MS analysis is complex, with numerous steps and varied protocols, resulting in sample variability in both quality and quantity. This kit has been designed to improve reproducibility while saving hands-on and processing time. The number of steps and processing time have been reduced through the addition of Universal Nuclease to reduce viscosity from nucleic acids (replacing sonication), a rapid 'one pot' reduction/alkylation solution for cysteine modification (carbamidomethylation, + 57.02), and a trypsin/Lys-C protease mix for more complete digestion. In addition, the kit includes Peptide Clean-up columns and buffers to prepare detergent-free peptide samples for MS analysis. The optimized reagents and protocol produce high-quality peptides that are compatible with TMT labeling and other downstream applications, or are ready for MS analysis.
• Optimized—streamlined protocol and reagents minimize the number of steps and time time needed to process samples
• Flexible—reagents and protocol have been verified using cells, plasma, and tissue samples for 10 to 100 μg samples
• Time-saving—sample processing time reduced from more than one day to less than three hours
• Compatible—final preparation is ready for direct MS analysis and other downstream applications, including TMT labeling
The sample preparation of peptides for MS analysis is complex, with numerous steps and varied protocols, resulting in sample variability in both quality and quantity. This kit has been designed to improve reproducibility while saving hands-on and processing time. The number of steps and processing time have been reduced through the addition of Universal Nuclease to reduce viscosity from nucleic acids (replacing sonication), a rapid 'one pot' reduction/alkylation solution for cysteine modification (carbamidomethylation, + 57.02), and a trypsin/Lys-C protease mix for more complete digestion. In addition, the kit includes Peptide Clean-up columns and buffers to prepare detergent-free peptide samples for MS analysis. The optimized reagents and protocol produce high-quality peptides that are compatible with TMT labeling and other downstream applications, or are ready for MS analysis.
For Research Use Only. Not for use in diagnostic procedures.
規格
DescriptionMS Sample Prep Kit
Final Product TypePeptides
For Use With (Equipment)Mass Spectrometer
Label or DyeUnlabeled
Quantity96 Reactions
Shipping ConditionWet Ice
Workflow StepProtein Digestion
Detection MethodMass Spectrometry
FormatKit
Product LineEasyPep
Product TypeSample Preparation Kit
Starting MaterialProtein Samples
Unit SizeEach
內容物與存放
- Lysis Solution, 25 mL, store at 4°C
- Universal Nuclease, 25 kU, store at -20°C
- Reduction Solution, 7 mL, store at 4°C
- Alkylation Solution, 7 mL, store at 4°C
- Enzyme Reconstitution solution, 7 mL, store at 4°C
- Pierce Trypsin/Lys-C Protease Mix, MS Grade, 5 x 100 μg, store at -20°C
- Digestion Stop Solution, 7 mL, store at 4°C
- Peptide Clean-Up Plate, 1 Ea., store at 4°C
- Wash and Elution Plate, 1 Ea., store at 4°C
- Sample Prep Plate, 1 Ea., store at 4°C
- Wash Solution A, 40 mL, store at 4°C
- Wash Solution B, 3 x 27 mL, store at 4°C
- Elution Solution, 2 x 20 mL, store at 4°C
引用資料與參考文獻 (4)
引用資料與參考文獻
Abstract
Discovery of Orally Available and Brain Penetrant AEP Inhibitors.
Journal:Journal of medicinal chemistry
PubMed ID:38090813
Alzheimer's Disease (AD) is the most widespread form of dementia, with one of the pathological hallmarks being the formation of neurofibrillary tangles (NFTs). These tangles consist of phosphorylated Tau fragments. Asparagine endopeptidase (AEP) is a key Tau cleaving enzyme that generates aggregation-prone Tau fragments. Inhibition of AEP to reduce the
Development of a First-in-Class RIPK1 Degrader to Enhance Antitumor Immunity.
Journal:Research square
PubMed ID:38659866
The scaffolding function of receptor interacting protein kinase 1 (RIPK1) confers intrinsic and extrinsic resistance to immune checkpoint blockades (ICBs) and has emerged as a promising target for improving cancer immunotherapies. To address the challenge posed by a poorly defined binding pocket within the intermediate domain, we harnessed proteolysis targeting
Proteomic Characterization of Transfusable Blood Components: Fresh Frozen Plasma, Cryoprecipitate, and Derived Extracellular Vesicles via Data-Independent Mass Spectrometry.
Journal:Journal of proteome research
PubMed ID:39254217
Extracellular vesicles (EVs) are a heterogeneous collection of particles that play a crucial role in cell-to-cell communication, primarily due to their ability to transport molecules, such as proteins. Thus, profiling EV-associated proteins offers insight into their biological effects. EVs can be isolated from various biological fluids, including donor blood components
Targeted sulfur(VI) fluoride exchange-mediated covalent modification of a tyrosine residue in the catalytic pocket of tyrosyl-DNA phosphodiesterase 1.
Journal:Communications chemistry
PubMed ID:39284936
Developing effective inhibitors of the DNA repair enzyme tyrosyl-DNA phosphodiesterase 1 (TDP1) has been challenging because of the enzyme shallow catalytic pocket and non-specific substrate binding interactions. Recently, we discovered a quinolone-binding hot spot in TDP1's active site proximal to the evolutionary conserved Y204 and F259 residues that position DNA.