Second Strand cDNA Synthesis Kit
Second Strand cDNA Synthesis Kit
Invitrogen™

Second Strand cDNA Synthesis Kit

Invitrogen Second Strand cDNA Synthesis Kit is designed to produce double-stranded cDNA from the first-strand reaction without the need for深入閱讀
Have Questions?
變更視圖buttonViewtableView
產品號碼No. of ReactionsVector
A48571200 Reactions
A4857050 Reactions
產品號碼 A48571
價格 (TWD)
117,320.00
Online offer
Ends: 31-Dec-2025
167,600.00
您節省 50,280.00 (30%)
Each
新增至購物車
No. of Reactions:
200 Reactions
價格 (TWD)
117,320.00
Online offer
Ends: 31-Dec-2025
167,600.00
您節省 50,280.00 (30%)
Each
新增至購物車
Invitrogen Second Strand cDNA Synthesis Kit is designed to produce double-stranded cDNA from the first-strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. The convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.

Features of the Second Strand cDNA Synthesis Kit include:
• Efficient synthesis of full-length double-stranded cDNA
• Fast—procedure completed in 1 hour
• Convenient—pre-mixed components
• Complete—includes residual RNA removal reagents

Applications
• Full-length double-stranded blunt-end cDNA synthesis for cloning
• Double-stranded cDNA library construction

規格
Final Product TypeDouble Stranded cDNA
Format200 reactions
No. of Reactions200 Reactions
Product TypecDNA Synthesis Kit
Quantity200 reactions
Reaction FormatSeparate Components
For Use With (Application)Cloning
Unit SizeEach
內容物與存放
Contents:
• Second Strand Enzyme Mix, 4 x 1 mL
• 5X Second Strand Reaction Mix, 4 x 250 μL
• 0.5 M EDTA, pH 8.0, 4 x 1 mL
• RNase I, 10 U/μL, 4 x 500 μL
• Water, nuclease-free, 12 x 1.25 mL

Storage: -15 to -25°C

常見問答集 (常見問題)

What is the correct reaction temperature that I should use for second strand cDNA synthesis using the Second Strand cDNA Synthesis Kit?

The second strand reaction must be incubated at 16 degrees C to prevent spurious synthesis by DNA polymerase I. At higher temperatures, DNA polymerase I tends to strand-displace rather than nick translate.

When using the Second Strand cDNA Synthesis Kit, what steps do I need to take to properly prepare the second strand reaction mixture?

Dilute the first-strand reaction as outlined in the manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0019413_SecondStrandcDNASynthesisKit_UG.pdf) on page 2 under Second Strand cDNA Synthesis step. Failure to properly dilute the first-strand reaction changes the pH of the second strand reaction and influences the activity of the 3'-5' and 5'-3' exonuclease activities of DNA polymerase I.

When using the Second Strand cDNA Synthesis Kit, how can I avoid incomplete RT inactivation?

Always perform the RT inactivation step after first-strand cDNA synthesis.

I am getting low yield of second strand cDNA product using the Second Strand cDNA Synthesis Kit. What is the reason?

The main reasons are incomplete RT inactivation, improper preparation of the second strand reaction mixture, and incorrect reaction temperature.