Ambion™ Poly(A) Polymerase catalyzes the addition of adenosine to the 3' end of RNA in a sequence-independent fashion. The enzyme深入閱讀
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產品號碼
Quantity
AM2030
80 units, 2 μL
產品號碼 AM2030
價格 (TWD)
7,150.00
線上優惠
Ends: 31-Dec-2025
10,200.00
您節省 3,050.00 (30%)
Each
新增至購物車
Quantity:
80 units, 2 μL
Request bulk or custom format
價格 (TWD)
7,150.00
線上優惠
Ends: 31-Dec-2025
10,200.00
您節省 3,050.00 (30%)
Each
新增至購物車
Ambion™ Poly(A) Polymerase catalyzes the addition of adenosine to the 3' end of RNA in a sequence-independent fashion. The enzyme can be used to append radiolabeled ATP to the 3' end of RNA molecules to generate labeled RNAs. One tube containing 80 U (2 U/ μL), 5X Poly(A) Polymerase Buffer, and 25 mM MnCl2 are provided. The enzyme can be used in conjunction with terminal transferase, reverse transcriptase, and thermostable polymerase to clone RNAs of unknown sequence.
Unit Definition: One unit is the amount of enzyme required to incorporate 1 nmol of AMP into acid-insoluble material in 10 min at 37°C.
For Research Use Only. Not for use in diagnostic procedures.
規格
Concentration5X
Quantity80 units, 2 μL
Shipping ConditionDry Ice
SourceRNA
PolymerasePoly(A) RNA Polymerase
Product LineAmbion
Unit SizeEach
內容物與存放
Poly(A) Polymerase, 5X Poly(A) Polymerase Buffer, and 25 mM MnCl2 should be stored at –20°C.
常見問答集 (常見問題)
Is the Poly(A) Polymerase (cloned) (Cat. No. AM2030) from E. coli?
PolyA polymerase (cloned) is purified from an E. coli strain overexpressing E. coli Poly(A) Polymerase I. Here is the link to the product information sheet: https://assets.thermofisher.com/TFS-Assets/LSG/manuals/4393885_PlyAPlymrseCln_PI.pdf