Fluorescein biotin was developed as an alternative to radioactive biotin for detecting and quantitating biotin-binding sites by either fluorescence or深入閱讀
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產品號碼
Quantity
B1370
5 mg
產品號碼 B1370
價格 (TWD)
10,010.00
Online offer
Ends: 31-Dec-2025
14,300.00
您節省 4,290.00 (30%)
Each
新增至購物車
Quantity:
5 mg
價格 (TWD)
10,010.00
Online offer
Ends: 31-Dec-2025
14,300.00
您節省 4,290.00 (30%)
Each
新增至購物車
Fluorescein biotin was developed as an alternative to radioactive biotin for detecting and quantitating biotin-binding sites by either fluorescence or absorbance. Upon binding to avidin or streptavidin, the fluorescence or absorbance of this molecule is quenched.
For Research Use Only. Not for use in diagnostic procedures.
規格
Label or DyeFITC (Fluorescein)
Product TypeMolecular Probes Fluorescein biotin
Quantity5 mg
Shipping ConditionRoom Temperature
Unit SizeEach
內容物與存放
Store at room temperature and protect from light.
引用資料與參考文獻 (17)
引用資料與參考文獻
Abstract
Functional consequences of insertions and deletions in the complementarity-determining regions of human antibodies.
Authors:Lantto J, Ohlin M
Journal:J Biol Chem
PubMed ID:12237318
'Insertions and deletions of nucleotides in the genes encoding the variable domains of antibodies are natural components of the hypermutation process, which may expand the available repertoire of hypervariable loop lengths and conformations. Although insertion of amino acids has also been utilized in antibody engineering, little is known about the ... More
Continuous-flow, on-line monitoring of biospecific interactions using electrospray mass spectrometry.
Authors:Hogenboom AC, de Boer AR, Derks RJ, Irth H
Journal:Anal Chem
PubMed ID:11534702
'A continuous-flow analytical screening system is presented using electrospray mass spectrometry to measure the interaction of biologically active compounds with soluble affinity proteins. The biochemical detection system is based on a solution-phase, homogeneous assay. In a first step, compounds to be screened (e.g., biotinylated compounds, concentration range 10-1,000 nmol/L) are ... More
Authors:Michael KL, Taylor LC, Schultz SL, Walt DR
Journal:Anal Chem
PubMed ID:9553489
'Array-based sensors provide an architecture for multianalyte sensing. In this paper, we report a new approach for array fabrication. Sensors are made by immobilizing different reactive chemistries on the surfaces of microspheres. Sensor arrays are prepared by randomly distributing a mixture of microsphere sensors on an optical substrate containing thousands ... More
Using affinity capillary electrophoresis to determine binding stoichiometries of protein-ligand interactions.
Authors:Chu YH, Lees WJ, Stassinopoulos A, Walsh CT
Journal:Biochemistry
PubMed ID:8075061
'We have developed a new method utilizing affinity capillary electrophoresis (ACE) for the determination of binding stoichiometries in biochemical systems. Using the same concentration of a ligand in the sample and the electrophoresis buffer, the appearance of an inverted peak corresponding to the free ligand in the resulting electropherogram provides ... More
Peptides, antibodies, and FRET on beads in flow cytometry: A model system using fluoresceinated and biotinylated beta-endorphin.
Authors:Buranda T, Lopez GP, Keij J, Harris R, Sklar LA
Journal:Cytometry
PubMed ID:10451503
'BACKGROUND: Particulate surfaces such as beads are routinely used as platforms for molecular assembly for fundamental and practical applications in flow cytometry. Molecular assembly is transduced as the direct analysis of fluorescence, or as a result of fluorescence resonance energy transfer. Binding of fluorescent ligands to beads sometimes alters their ... More