Biocytin (ε-Biotinoyl-L-Lysine)
Biocytin (ε-Biotinoyl-L-Lysine)
引用資料與參考文獻 (174)
Invitrogen™

Biocytin (ε-Biotinoyl-L-Lysine)

Biocytin can be used as microinjectable anterograde and transneuronal tracers that is fixable with aldehyde-based fixatives. Additionally the primary amine深入閱讀
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產品號碼Quantity
B1592100 mg
產品號碼 B1592
價格 (TWD)
***
新增至購物車
Quantity:
100 mg
價格 (TWD)
***
新增至購物車
Biocytin can be used as microinjectable anterograde and transneuronal tracers that is fixable with aldehyde-based fixatives. Additionally the primary amine on the lysine can be reversibly coupled to aldehydes and ketones to form a Schiff base - which can be reduced to a generate stable amine derivative by sodium borohydride (NaBH4) or sodium cyanoborohydride (NaCNH3). Carboxylic acids of proteins and other water-soluble biopolymers can be coupled to this molecule in aqueous solution using water-soluble carbodiimides such as EDAC (E2247).
For Research Use Only. Not for use in diagnostic procedures.
規格
Label TypeBiotin & Other Haptens
Product TypeBiocytin
Quantity100 mg
Reagent TypeBiocytins
Shipping ConditionRoom Temperature
Unit SizeEach
內容物與存放
Store at room temperature.

引用資料與參考文獻 (174)

引用資料與參考文獻
Abstract
Authors:
Journal:
PubMed ID:7905516
Human serum biotinidase. cDNA cloning, sequence, and characterization.
Authors:Cole H, Reynolds TR, Lockyer JM, Buck GA, Denson T, Spence JE, Hymes J, Wolf B
Journal:J Biol Chem
PubMed ID:7509806
Biotinidase (EC 3.5.1.12) catalyzes the hydrolysis of biocytin, the product of biotin-dependent carboxylase degradation, to biotin and lysine. Biotinidase deficiency is an inherited metabolic disorder of biotin recycling that is characterized by neurological and cutaneous abnormalities, and can be successfully treated with biotin supplementation. Sequences of tryptic peptides of the ... More
Purification of biotinidase from human plasma and its activity on biotinyl peptides.
Authors:Craft DV, Goss NH, Chandramouli N, Wood HG
Journal:Biochemistry
PubMed ID:3925986
Biotinidase catalyzes the hydrolysis of N epsilon-biotinyllysine (biocytin) to form biotin and free lysine. The enzyme has been purified 4800-fold from outdated human plasma and was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to have a molecular weight of (76 +/- 2) X 10(3). The same molecular weight was found ... More
Deformation of network connectivity in the inferior olive of connexin 36-deficient mice is compensated by morphological and electrophysiological changes at the single neuron level.
Authors:De Zeeuw CI, Chorev E, Devor A, Manor Y, Van Der Giessen RS, De Jeu MT, Hoogenraad CC, Bijman J, Ruigrok TJ, French P, Jaarsma D, Kistler WM, Meier C, Petrasch-Parwez E, Dermietzel R, Sohl G, Gueldenagel M, Willecke K, Yarom Y
Journal:J Neurosci
PubMed ID:12805309
Compensatory mechanisms after genetic manipulations have been documented extensively for the nervous system. In many cases, these mechanisms involve genetic regulation at the transcription or expression level of existing isoforms. We report a novel mechanism by which single neurons compensate for changes in network connectivity by retuning their intrinsic electrical ... More
Structure and function of retinal ganglion cells innervating the cat's geniculate wing: an in vitro study.
Authors:Pu M, Berson DM, Pan T
Journal:J Neurosci
PubMed ID:8027783
'We have examined in vitro the morphology and visual response properties of retinal ganglion cells innervating a component of the cat's lateral geniculate nucleus known as the geniculate wing (or retinorecipient zone of the pulvinar). Ganglion cells were first labeled in situ by retrograde transport of fluorescent microspheres from the ... More
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