ChargeSwitch™ gDNA Buccal Cell Kit

Sample size 1 buccal swab; Typical performance up to 6 μg• Maximum yield sensitivity• Rapid single-tube protocol, suitable for automation•深入閱讀

產品號碼	No. of Reactions
CS11021	50 Preps

產品號碼 CS11021

價格 (TWD)

新增至購物車

No. of Reactions:

50 Preps

價格 (TWD)

新增至購物車

Sample size 1 buccal swab; Typical performance up to 6 μg

• Maximum yield sensitivity
• Rapid single-tube protocol, suitable for automation
• High quality DNA for downstream Single Tandem Repeat (STR) analysis

The ChargeSwitch™ gDNA Buccal Cell Kit maximizes the yield of high quality genomic DNA from buccal samples for rapid buccal swab processing. This kit includes all the necessary reagents for the number of preps listed and requires the use of a magnetic accessory. Please see the Accessories Chapter (Chapter 6) to select the best magnetic accessory for your needs.

For Research Use Only. Not for use in diagnostic procedures.

Cell TypeBuccal Cell

For Use With (Application)Forensic

High-throughput CompatibilityHigh-throughput Compatible

No. of Reactions50 Preps

Product LineChargeSwitch

Product TypegDNA Buccal Cell Kit

Quantity50 Preps

Sample TypeBuccal Samples

Shipping ConditionRoom Temperature

TargetGenomic DNA

FormatKit

Isolation TechnologyChargeSwitch Chemistry

Unit SizeEach

內容物與存放

All components of the ChargeSwitch™ gDNA Buccal Cell Kits are shipped at room temperature. Upon receipt, store the Proteinase K at 4°C. Store all other components at room temperature. All components are guaranteed stable for 6 months, if stored properly.

Components:
• ChargeSwitch™ Lysis Buffer (L11)
• ChargeSwitch™ Magnetic Beads
• Proteinase K (20 mg/mL in 50 mM Tris-HCl, pH 8.5, 5 mM CaCl2, 50% glycerol)
• ChargeSwitch™ Purification Buffer (N6)
• ChargeSwitch™ Wash Buffer (W12)
• ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)

常見問答集 (常見問題)

I used water to elute my plasmid instead of the Elution Buffer with my ChargeSwitch kit, but saw very low plasmid yield. Why is this?

We strongly recommend using the Elution Buffer provided in the kit, and do not recommend elution with water. If you need to elute in any other buffer, be sure to use a buffer of pH 8.5-9.0 for efficient DNA elution.

What is the difference between the ChargeSwitch Pro kit and the Charge-Switch Pro filter kit?

The filter kit is based upon a dual nested column design, which facilitates the fastest and simplest protocol for obtaining high quality plasmid (less than 10 EU/µg DNA). Modified wash buffer 1 removes more endotoxin.

What is the typical yield when using the ChargeSwitch Pro Filter Plasmid Mini or Midi Kits?

The typical yield is 25 µg from a 5 mL culture for the ChargeSwitch Pro mini kit, 200 µg from a 25 mL culture for the midi kit, and 800 µg from a 100 mL culture from the maxi kit. The plasmid size range for the kits is typically 3-9 kb.

I want to isolate genomic DNA from buccal cells. How do I do this?

We would recommend using one of our MagMAX DNA Multi-Sample Ultra 2.0 Kit or ChargeSwitch gDNA Buccal Cell Kits, which were designed to isolate high-quality genomic DNA from buccal samples. We also offer normalized kits, which will normalize isolated DNA concentration from sample to sample.

How does ChargeSwitch technology work?

Based on a patented switchable chemistry, ChargeSwitch purification utilizes the charge on a solid surface for nucleic acid capture. At lower pH, the surface chemistry holds a positive charge and attracts nucleic acid. At higher pH, the surface chemistry is neutral and any bound nucleic acid is released.