EnzChek™ Protease Assay Kit, red fluorescence, 100-1000 assays

引用資料與參考文獻 (9)

Invitrogen™

EnzChek™ Protease Assay Kit, red fluorescence, 100-1000 assays

Detects metallo-, serine, acid, and sulfhydryl proteases.

產品號碼	Quantity
E6639	1 Kit

產品號碼 E6639

價格 (TWD)

Quantity:

1 Kit

價格 (TWD)

The EnzChek™ Protease Assay Kit, red fluorescence, is a fast, simple, and direct fluorescence-based assay for detecting metallo-, serine, acid, and sulfhydryl proteases. The accompanying increase in fluorescence, which can be measured with a fluorescence microplate reader, is proportional to protease activity.

Generalized mechanism allows for measurement of a variety of proteases
Can be used to continuously measure protease kinetics
Excitation/emission similar to Texas Red™ dye (589/617 nm)

This EnzChek™ Protease Assay Kit contains a casein derivative that has been extensively labeled with the red-fluorescent BODIPY™ TR-X dye, which results in a quenching of the fluorescent dye. Protease-catalyzed hydrolysis releases the highly-fluorescent BODIPY™ TR-X dye-labeled peptides, allowing for quantitative detection of protease activity in solution. The red-fluorescent BODIPY™ TR-X dye has excitation and emission spectra similar to those of the Texas Red™ fluorophore.

For Research Use Only. Not for use in diagnostic procedures.

Detection MethodFluorescence

Emission Wavelength617 nm

Excitation Wavelength589 nm

Quantity1 Kit

Shipping ConditionRoom Temperature

Substrate PropertiesProtein-Based Substrate

Substrate TypeProtease Substrate

Target EnzymeProteases

For Use With (Application)Enzyme and Metabolic Activity

For Use With (Equipment)Microplate Reader

Product LineEnzChek

Product TypeEnzChek Assay Kit

Unit SizeEach

內容物與存放

Store in freezer -5°C to -30°C and protect from light.

常見問答集 (常見問題)

What is the optimal pH range for protease activity detection using the EnzChek Protease Assay Kit?

The digestion buffer provided in the kit (pH 7.8) is recommended for detecting the protease activity of most proteolytic enzymes with activity optima from pH 7.4 to 8.0. However, if you are working with an enzyme that requires activation compounds or a unique pH environment, then prepare the specific buffer required in place of the digestion buffer.

What is the shelf life for EnzChek Protease Assay kits (Cat. Nos. E6638, E6639)?

As stated in the manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/mp06638.pdf) on page 2 under section "Storage and Handling", these kits are stable for 6-12 months if stored as indicated.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How long is the reconstituted BODIPY substrate in the EnzChek Protease Assay Kit, red fluorescence, 100-1000 assays (Cat. No. E6639) stable?

Reconstituted BODIPY casein substrates may be stored at 4 degrees C for 2-4 weeks. We recommend the addition of sodium azide at a final concentration of 2 mM to act as a preservative. If longer storage is required, freeze at -20 degrees C. Protect from light and avoid repeated freezing and thawing.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用資料與參考文獻 (9)

引用資料與參考文獻

Abstract

Quenched BODIPY dye-labeled casein substrates for the assay of protease activity by direct fluorescence measurement.

Authors:Jones LJ, Upson RH, Haugland RP, Panchuk-Voloshina N, Zhou M, Haugland RP

Journal:Anal Biochem

PubMed ID:9299009

'We have prepared casein conjugates of two BODIPY dyes for use as fluorogenic protease substrates in homogeneous assays. Both conjugates are labeled to such an extent that the dyes are efficiently quenched in the protein, yielding virtually nonfluorescent substrate molecules. These fluorogenic substrates release highly fluorescent BODIPY dye-labeled peptides upon ... More

Subnanoliter enzymatic assays on microarrays.

Authors:Angenendt P, Lehrach H, Kreutzberger J, Glökler J

Journal:Proteomics

PubMed ID:15700241

'Many areas of research today are based on enzymatic assays most of which are still performed as enzyme-linked immunosorbent assays in microtiter plates. The demand for highly parallel screening of thousands of samples eventually led to a miniaturization and automation of these assays. However, the final transfer of enzymatic assays ... More

Continuous assay of proteases using a microtiter plate fluorescence reader.

Authors:Menges DA, Ternullo DL, Tan-Wilson AL, Gal S

Journal:Anal Biochem

PubMed ID:9398357

A protease-dependent mechanism for initiating T-dependent B cell responses to large particulate antigens.

Authors:Catron DM, Pape KA, Fife BT, van Rooijen N, Jenkins MK,

Journal:J Immunol

PubMed ID:20208013

Ab production is critical for antimicrobial immunity, and the initial step in this process is the binding of Ag to the BCR. It has been shown that small soluble proteins can directly access the lymph node follicles to reach naive B cells, but virus particles must be translocated into follicles ... More

Selective modulation of superantigen-induced responses by streptococcal cysteine protease.

Authors:Kansal RG, Nizet V, Jeng A, Chuang WJ, Kotb M

Journal:J Infect Dis

PubMed ID:12552423

Streptococcal pyrogenic exotoxin (Spe) B, a streptococcal cysteine protease, is believed to be important in group A streptococcal (GAS) pathogenesis. The present study examined the effect of SpeB on the activity of superantigenic exotoxins secreted by M1T1 GAS isolates. The proliferative response of human lymphocytes to culture supernatant (SUP) from ... More

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