T4 RNA Ligase (10 U/μL)
T4 RNA Ligase (10 U/μL)
Thermo Scientific™

T4 RNA Ligase (10 U/μL)

Thermo Scientific T4 RNA Ligase catalyzes the ATP-dependent intra- and intermolecular formation of phosphodiester bonds between 5'-phosphate and 3'-hydroxyl termini深入閱讀
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產品號碼Quantity
EL00211,000 units
產品號碼 EL0021
價格 (TWD)
3,420.00
Online offer
Ends: 31-Dec-2025
4,880.00
您節省 1,460.00 (30%)
Each
新增至購物車
Quantity:
1,000 units
Request bulk or custom format
價格 (TWD)
3,420.00
Online offer
Ends: 31-Dec-2025
4,880.00
您節省 1,460.00 (30%)
Each
新增至購物車
Thermo Scientific T4 RNA Ligase catalyzes the ATP-dependent intra- and intermolecular formation of phosphodiester bonds between 5'-phosphate and 3'-hydroxyl termini of oligonucleotides, single-stranded RNA and DNA.

The minimal substrate is a nucleoside 3',5'-biphosphate in intermolecular reaction and oligonucleotide of 8bases in intramolecular reaction.

Applications

• RNA 3'-end labeling with cytidine 3',5'-bis [alpha-32P] phosphate
• Joining RNA to RNA
• Synthesis of oligoribonucleotides and oligodeoxyribonucleotides
• Specific modifications of tRNAs
• Oligodeoxyribonucleotide ligation to single-stranded cDNAs for 5' RACE (Rapid Amplification of cDNA Ends)
• Site-specific generation of composite primers for PCR

Note

The recommended BSA concentration in the reaction mixture is 0.1mg/mL.
For Research Use Only. Not for use in diagnostic procedures.
規格
Compatible Buffer10X Reaction Buffer
Product TypeT4 RNA Ligase
Quantity1,000 units
Concentration10 U/μL
EnzymeT4 RNA Ligase
Unit SizeEach

常見問答集 (常見問題)

What are some of the problems associated with sticky-end cloning?

The amplified DNA needs to be purified from the PCR mixture components prior to cloning. The dNTPs carried over from the PCR are competitive inhibitors for ATP in the ligation reaction.

If during synthesis of the PCR primers their chemical integrity has been compromised by either a base substitution or modification, the enzyme recognition site may in actuality not exist. If this is the case, PCR products will be resistant to digestion with restriction enzymes. It may be necessary to use a higher concentration of the restriction enzyme and to incubate at the appropriate temperature overnight to ensure cutting.