T4 beta-glucosyltransferase

Thermo Scientific T4 β-glucosyltransferase (T4 BGT) transfers the glucose moiety of uridine diphosphoglucose (UDP-glucose) to the 5-hydroxymethylcytosine (5-hmC) residues in double-stranded DNA generating ß-glucosyl-5 hydroxymethylcytosine.

Thermo Scientific T4 BGT is specifically formulated for fast reaction times without compromising the reaction efficiency. The enzyme completes 5-hmC glucosylation of 1 μg DNA at 37°C in 15 minutes.

Highlights

• Specific—selectively transfers glucose to the hydroxymethyl moiety of 5-hmC
• Fast—complete glucosylation of 1 μg DNA in just 15 min
• Convenient—supplied with optimized buffer and UDP-glucose.

Applications

• Locus specific detection of 5-hmC
• Enrichment of 5-hmC containing DNA
• Labeling of 5-hmC residues using a radioactive UDP-glucose donor

Includes
• T4 β-glucosyltransferase, 5 U/μL
• 10X Epi buffer
• 10X UDP-glucose