Thermo Scientific™

Phire Hot Start II DNA Polymerase

產品號碼: F122L
Thermo Scientific™

Phire Hot Start II DNA Polymerase

產品號碼: F122L
產品號碼
F122L
包裝規格
1,000 reactions
價格 (TWD)
價格 36,500.00
線上優惠 29,200.00
您的價格
供應狀況
-
數量
產品號碼
包裝規格
200 reactions
價格 (TWD)
價格 8,840.00
線上優惠 6,188.00
(01-Jul-2024 結束)
您的價格
供應狀況
-
數量
產品號碼包裝規格價格 (TWD)供應狀況數量
F122L1,000 reactions
價格 36,500.00
線上優惠 29,200.00
您的價格
-
F122S200 reactions
價格 8,840.00
線上優惠 6,188.00
(01-Jul-2024 結束)
您的價格
-
產品概覽
圖表
影片
建議
建議
文件
常見問題
引用資料與參考文獻
其他資訊
建議
Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications. It outperforms every Taq-based hot start polymerase on the market. Phire Hot Start II DNA Polymerase is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields. These features are achieved through advanced protein engineering of the polymerase. It incorporates a unique double-stranded DNA binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to Taq DNA polymerase.

The hot start modification of Phire Hot Start II DNA Polymerase is based on the Affibody inactivation technology. This technology increases the specificity of the PCR reaction with no additional time required for initial activation of the enzyme.

Highlights

Quick hot start—No reactivation step
Fast enzyme—Amplify four times faster than with hot start Taq
Robust—Minimal reaction optimization due to high inhibitor tolerance
High yields—Abundant products due to high efficiency
Longer PCR products—Amplify significantly longer DNA fragments than with any hot start Taq

Applications

• Hot Start PCR
• Routine PCR
• Non-high fidelity PCR
• Fast PCR
• High throughput PCR
• Genotyping

Note:The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases. For optimal results start by accurately calculating your Tm with our Tm calculator.

Phire Green Hot Start II DNA Polymerase is also available (F-124S or F-124L) for direct loading of PCR products on gel.
For Research Use Only. Not for use in diagnostic procedures.

規格

GC-Rich PCR Performance
Medium
Polymerase
Phire Hot Start II DNA polymerase
Reaction Speed
Fast
Product Line
Phire
Product Type
DNA Polymerase
Purity or Quality Grade
DHPLC
For Use With (Application)
Cloning
Concentration
50X
Fidelity (vs. Taq)
2 X
Hot Start
Built-In Hot Start
No. of Reactions
1000 Reactions
Overhang
Blunt
Reaction Format
Standalone
Starting Material
DNA

內容物與存放

• 1 x 1 mL Phire Hot Start II DNA Polymerase
• 7 x 1.5 mL 5X Phire Reaction Buffer (provides 1.5 mM MgCl2)
• 1 x 500 μL DMSO

Store at -20°C.

圖表

文件與下載

證書

    常見問答集 (常見問題)

    引用資料與參考文獻

    Search citations by name, author, journal title or abstract text