IC Fixation Buffer

Intracellular fixation buffer for use in flow cytomerty, particularly for fixation in intracellular staining procedures. Composition is paraformaldehyde in phosphate深入閱讀

產品號碼	Quantity
FB001	100 mL

產品號碼 FB001

價格 (TWD)

新增至購物車

Quantity:

100 mL

價格 (TWD)

新增至購物車

Intracellular fixation buffer for use in flow cytomerty, particularly for fixation in intracellular staining procedures. Composition is paraformaldehyde in phosphate buffered saline, pH 7.3.

These Made-to-Order catalog items have a 2 week lead time.

For Research Use Only. Not for use in diagnostic procedures.

For Use With (Application)Cell Surface Antibodies, Intracellular Cytokine Antibodies

Quantity100 mL

Shipping ConditionWet Ice

Product TypeFixation Buffer

Solution TypeFixation Buffer

pH7.3

Unit SizeEach

內容物與存放

Store at 2°C to 8°C. Do not freeze.

常見問答集 (常見問題)

What fixative should I use to fix my cells or tissues for antibody labeling?

Aldehyde-based fixatives (e.g., formaldehyde, glutaraldehyde) crosslink various cellular components, which helps retain proteins and cell morphology, but some antigens can be masked by the crosslinking, requiring antigen retrieval methods to unmask the antigen binding sites. Also, aldehyde-based fixation requires permeabilization to allow entry of antibodies. Organic solvents such as methanol and acetone condense proteins and permeabilize the cells, but cell morphology can be compromised. Surface antigens can be labeled without fixation.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.