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引用資料與參考文獻 (8)
Invitrogen™
Guanosine 5'-Triphosphate, BODIPY™ FL 2'-(or-3')-O-(N-(2-Aminoethyl) Urethane), Trisodium Salt (BODIPY™ FL GTP)
BODIPY FL adenosine 5'-triphosphate (A-12410) and BODIPY FL guanosine 5'-triphosphate are composed of a BODIPY FL fluorophore attached to the深入閱讀
| 產品號碼 | Quantity |
|---|---|
| G12411 | 100 μL |
產品號碼 G12411
價格 (TWD)
11,830.00
Online offer
Ends: 31-Dec-2025
16,900.00您節省 5,070.00 (30%)
Each
Quantity:
100 μL
價格 (TWD)
11,830.00
Online offer
Ends: 31-Dec-2025
16,900.00您節省 5,070.00 (30%)
Each
BODIPY FL adenosine 5'-triphosphate (A-12410) and BODIPY FL guanosine 5'-triphosphate are composed of a BODIPY FL fluorophore attached to the 2' or 3' position of the ribose ring via a linker. Interactions between the fluorophore and the purine base are evident from the spectroscopic properties of these nucleotide analogs. The fluorescence quantum yield of BODIPY FL GTP is only about one-tenth that of BODIPY FL ATP; however, this yield may be enhanced upon binding of BODIPY FL GTP to GTP-binding proteins.
For Research Use Only. Not for use in diagnostic procedures.
規格
Label or DyeBODIPY™ FL
Product TypeGuanosine 5'-Triphosphate
Quantity100 μL
Shipping ConditionWet Ice
Concentration5 mM
Product LineBODIPY
Unit SizeEach
內容物與存放
Store in freezer -5°C to -30°C and protect from light.
引用資料與參考文獻 (8)
引用資料與參考文獻
Abstract
Capillary electrophoresis assay for G protein-coupled receptor-mediated GTPase activity.
Journal:Anal Chem
PubMed ID:17263349
'We describe a capillary electrophoresis (CE) assay to detect G protein-coupled receptor (GPCR)-stimulated G protein GTPase activity in cell membranes expressing alpha2A adrenoreceptor-Galphao1 wild-type (wt) or C351I mutant fusion proteins using a fluorescent, hydrolyzable GTP analogue. As no change in total fluorescence is observed by conversion of substrate to product,
Automated single-cell electroporation.
Journal:Biotechniques
PubMed ID:17068953
Identification of a small GTPase inhibitor using a high-throughput flow cytometry bead-based multiplex assay.
Journal:J Biomol Screen
PubMed ID:20008126
Small GTPases are key regulators of cellular activity and represent novel targets for the treatment of human diseases using small-molecule inhibitors. The authors describe a multiplex, flow cytometry bead-based assay for the identification and characterization of inhibitors or activators of small GTPases. Six different glutathione-S-transferase (GST)-tagged small GTPases were bound
A direct fluorescence-based assay for RGS domain GTPase accelerating activity.
Journal:Anal Biochem
PubMed ID:15840508
Diverse extracellular signals regulate seven transmembrane-spanning receptors to modulate cellular physiology. These receptors signal primarily through activation of heterotrimeric guanine nucleotide binding proteins (G proteins). A major determinant of heterotrimeric G protein signaling in vivo and in vitro is the intrinsic GTPase activity of the Galpha subunit. RGS (regulator of
Real-time detection of basal and stimulated G protein GTPase activity using fluorescent GTP analogues.
Journal:J Biol Chem
PubMed ID:15613467
Hydrolysis of fluorescent GTP analogues BODIPY FL guanosine 5 '-O-(thiotriphosphate) (BGTPgammaS) and BODIPY FL GTP (BGTP) by Galpha(i1) and Galpha was characterized using on-line capillary electrophoresis (o) laser-induced fluorescence assays in order that changes in sub-strate, substrate-enzyme complex, and product could be monitored separately. Apparent k values (V /[E]) (max