TRIS-glycine-SDS running buffer (10X), pH 8.5
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TRIS-glycine-SDS running buffer (10X), pH 8.5
Thermo Scientific Chemicals

TRIS-glycine-SDS running buffer (10X), pH 8.5

TRIS-glycine-SDS running buffer (10X), suitable for protein gel analysis, is used in several research areas, including biochemistry, molecular biology, cell biology, and immunology.
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產品號碼 J61006.K7
亦稱為 J61006-K7
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4 L
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規格
Chemical Name or MaterialTRIS-glycine-SDS running buffer (10X)
PackagingPlastic bottle
Recommended StorageAmbient temperatures
Solubility InformationIt is soluble in water.
TSCAYes
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Tris-Glycine SDS Running Buffer (10X) is used as the electrophoresis buffer during the stacking and resolve process of sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE). It is ideally suited for protein gel analysis in biochemical, molecular biological, cell biological,and immunological research studies.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

• TRIS-glycine-SDS running buffer (10X), is the most commonly used buffer for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of proteins

Application

• This solution is the most commonly used buffer for protein gel analysis in several research studies
• It is also used to block membranes with 5% non-fat dry milk for western blotting
• TRIS-glycine-SDS running buffer is commonly used as both anode buffer and cathode buffer
• The use of SDS in the buffer helps keep the proteins linear. Glycine is an amino acid whose charge state plays an important role in the stacking gel

RUO – Research Use Only

General References:

  1. L Zhang.; R Hernan.; B Brizzard. Multiple tandem epitope tagging for enhanced detection of protein expressed in mammalian cells. Molecular biotechnology. 2001, 19, (3), 680 - 685.
  2. B Knebelmann.; S Ananth.; HT Cohen.; VP Sukhatme. Transforming growth factor α is a target for the von Hippel-Lindau tumor suppressor. Cancer research. 1998, 58, 226 - 230.

常見問答集 (常見問題)

What are the concentrations of Tris base, glycine and SDS in the TRIS-glycine-SDS running buffer (10X), pH 8.3?

The TRIS-glycine-SDS running buffer (10X), pH 8.3 contains 0.25 M Tris base, 1.92 M glycine, and 1% SDS.