Deoxyribonuclease I, bovine pancreas, Purified

Name: Deoxyribonuclease I, bovine pancreas, Purified
SKU: J61061.LB0

Deoxyribonuclease I, CAS # 9003-98-9, also known as DNase I, is an endonuclease isolated from bovine pancreas used mainly to remove deoxyribonucleic acid (DNA) from protein samples.

變更視圖

Quantity:

5 mg

20 mg

100 mg

嘗試 chem dex^TM 網路應用程式

This chemical may require us to obtain additional information for our regulatory and chemical compliance records. If required, we will contact you for this information once your order is placed.

產品號碼 J61061.LB0

亦稱為 J61061-#0

價格 (TWD)

Quantity:

5 mg

Request bulk or custom format

化學識別

CAS9003-98-9

IUPAC NameDeoxyribonuclease

SMILES*

MDL NumberMFCD00130918

規格

規格表

Unit definitionOne unit causes an increase in absorbance at 260nm of 0.001 per minute at 25?C, pH 5.0 when acting on highly polymerized DNA in the presence of magnesium and calcium

UV/VisA280 (1mg/ml): Report value

FormPowder

Assay (special)Endotoxin (EU/mg): Report value

units/mgdw≥ 2,000 Units/mg dry weght

Deoxyribonuclease I, is used in RNA preparations from tissue or bacterial cell cultures. Deoxyribonuclease is used to remove DNA from protein samples, It is also used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. It is used in investigating the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

Deoxyribonuclease I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5'-phosphates. In the presence of Mg2+, this compound cleaves each strand of DNA independently

Application

In biomedical research, this compound is used to nick DNA as a first step to integrate labeled bases into DNA
It can be used to eliminate genomic DNA from RNA solutions before RT-PCR
It is also used during isolation and purification of plasma membrane vesicles from Neurospora crassa cell culture
It can decrease viscosity in cell culture lysates, providing better yields in primary cell isolation
It is also commonly used in combination with trypsin for the preparation of single cell suspension from rats
Deoxyribonuclease I, bovine pancreas has been used in some studies in order to compare different methods for reducing RNase contamination in preparations of DNase
This compound can be applied in studies to investigate the impact of the composition of sodium dodecyl sulfate (SDS) preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis

RUO – Research Use Only

General References:

G A Scarborough. The neurospora plasma membrane ATPase is an electrogenic pump. PNAS. 1976, 73 (5), 1485-1488.
U Lindberg. Molecular weight and amino acid composition of deoxyribonuclease I. Biochemistry. 1967, 6 (1), 335-342.