β-Gal Assay Kit
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Invitrogen™

β-Gal Assay Kit

The β-Gal Assay Kit provides the reagents required to quickly measure the levels of active β-galactosidase expressed in cells transfected深入閱讀
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產品號碼Quantity
K14550180 mL
產品號碼 K145501
價格 (TWD)
***
新增至購物車
Quantity:
80 mL
價格 (TWD)
***
新增至購物車
The β-Gal Assay Kit provides the reagents required to quickly measure the levels of active β-galactosidase expressed in cells transfected with plasmids expressing the lacZ gene. lacZ is a bacterial gene often used as a reporter construct in eukaryotic transfection experiments. The gene product of lacZ, β-galactosidase, is resistant to proteolysis in cellular lysates and its activity is easily assayed. β-galactosidase catalyzes the hydrolysis of β-galactosidase such as ortho-nitrophenyl-D-galactopyranoside (ONPG). Hydrolysis of ONPG to the ONP anion produces a bright yellow color with a peak absorbance at 420 nm that can be quantified using a spectrophotometer.
10.7 gene expression
For Research Use Only. Not for use in diagnostic procedures.
規格
Assayβ-Gal (lacZ) Assay
Product TypeBeta-Gal Assay Kit
Quantity80 mL
SubstrateONPG
TargetBeta-Galactosidase
Detection MethodColorimetric
Unit SizeEach
內容物與存放
The β-Gal Assay Kit contains 10X PBS (phosphate buffered saline), lysis buffer, cleavage buffer, β-mercaptoethanol, ONPG, stop buffer. Store ONPG at -20°C. All other components are stored at either room temperature or +4°C. All reagents are guaranteed stable for 6 months when properly stored.

常見問答集 (常見問題)

Are detergents compatible with the β-Gal (ONPG) assay kit (K1455-01)?

Neither NP40 nor Triton X-100 (1%) should interfere with the reaction, although there is a chance that a precipitate may form when the detergent is added to the b-Gal assay reagent.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

How do I resuspend IPTG and X-Gal?

IPTG can be reconstituted in water. Make a stock of 100 mM in water and store working aliquots at -20°C. X-gal can be reconstituted in DMSO, or in a 50:50 mix of DMSO and water. To do the latter, you must dissolve in DMSO first, and then add water to bring up to final volume. It is not necessary to filter sterilize these solutions.

The X-gal solution should be protected from light. To make plates, add 50 ug/ml X-gal and 1 mM (0.24 mg/mL) IPTG to LB/agar that has been cooled down to 50°C. To spread on top of plates, use 50 µl 2% stock of X-gal and 30 µl 100 mM stock of IPTG. 

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.