S.N.A.P.™ Plasmid DNA MidiPrep Kit
Product Image
Invitrogen™

S.N.A.P.™ Plasmid DNA MidiPrep Kit

The S.N.A.P.™ MidiPrep Kit is a medium-scale plasmid DNA purification kit designed for fast and easy purification of pure plasmid深入閱讀
Have Questions?
產品號碼Quantity
K19100120 preps
產品號碼 K191001
價格 (TWD)
20,300.00
Online offer
Ends: 31-Mar-2026
29,000.00
您節省 8,700.00 (30%)
Each
新增至購物車
Quantity:
20 preps
價格 (TWD)
20,300.00
Online offer
Ends: 31-Mar-2026
29,000.00
您節省 8,700.00 (30%)
Each
新增至購物車
The S.N.A.P.™ MidiPrep Kit is a medium-scale plasmid DNA purification kit designed for fast and easy purification of pure plasmid DNA from 10-100 ml of overnight culture (Figure 1). The S.N.A.P.™ MidiPrep procedure takes just 40 minutes. You simply:

• Lyse cells and filter debris with the S.N.A.P.™ filtering column
• Apply flow-through to the S.N.A.P.™ DNA binding column
• Wash the column
• Elute pure, supercoiled plasmid DNA in water or TE buffer

Plasmid DNA purified with the S.N.A.P.™ MidiPrep Kit is ready to use right off the column. No precipitation is necessary. Plasmid is eluted in TE or water at ∼200 ng/μl-perfect for subsequent applications including mammalian cell transfection. Typical yields are shown in Table 1.
For Research Use Only. Not for use in diagnostic procedures.
規格
Final Product TypePlasmid DNA
For Use With (Application)Sequencing, Cloning, Transformation, Transfection, In Vitro Transcription
High-throughput CompatibilityNot High-throughput Compatible (Manual)
Prep Scale100-200 μg (Medium-Scale) Plasmid DNA
Product LineS.N.A.P.
Product TypePlasmid DNA MidiPrep Kit
Quantity20 preps
Sample TypeBacterial Culture
ScaleMidi
TargetPlasmid DNA
Test Time40 min.
FormatKit
Isolation TechnologySpin Column
Unit SizeEach
內容物與存放
Store the S.N.A.P.™ MidiPrep filtering columns, S.N.A.P.™ MidiPrep DNA binding columns, lysis buffer, binding buffer, wash buffer, and 4X final wash at room temperature. Store the resuspension buffer, precipitation salt, and RNase A at +4°C. All reagents are guaranteed stable for 6 months when properly stored.

常見問答集 (常見問題)

Which centrifuge is used at Thermo Fisher Scientific for the S.N.A.P. MidiPrep protocol?

The Sorval RT6000B table-top centrifuge is used at Thermo Fisher Scientific for the S.N.A.P. MidiPrep protocol. The low-speed spins are around 2,000 rpm and the high-speed spins are around 3,000 rpm. You may also use the Sorval RC-5B centrifuge with the SS-34 rotor. However, you might observe some spillage with this centrifuge.

What is the upper size limit for plasmid purification with the S.N.A.P. Miniprep Kit?

Bacmid DNA (>100 kbp) has been purified with the S.N.A.P. miniprep kit.

The PureLink HiPure Plasmid DNA Purification Kits (mini/midi/maxi scales) can also be used for large constructs. The HiPure kit manuals contain a modified protocol designed to help increase yields with these large plasmid isolations.

What are the compositions of the S.N.A.P. MiniPrep and MidiPrep Kit solutions?

The formulations are published in the manual for the S.N.A.P. MidiPrep kit, and are the same as the ones used in the MiniPrep kit.

What is the resin in the S.N.A.P. kits?

The column contains a silica/glass fiber based resin. However, the exact composition is proprietary.

Why is LB medium recommended for growing up plasmid preps in the S.N.A.P. protocol?

The volumes of culture recommended in our S.N.A.P. kits assume an OD of no more than 2.0. If a rich medium is used, the volume processed should be reduced to avoid overloading the column.