LIVE/DEAD™ Cell-Mediated Cytotoxicity Kit, for animal cells
LIVE/DEAD™ Cell-Mediated Cytotoxicity Kit, for animal cells
引用資料與參考文獻 (14)
Invitrogen™

LIVE/DEAD™ Cell-Mediated Cytotoxicity Kit, for animal cells

The LIVE/DEAD® Cell-Mediated Cytotoxicity Kit measures natural killer (NK) cell-mediated, lymphokine-activated killer (LAK) cell-mediated and T cell mediated cytotoxicity. Target深入閱讀
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產品號碼Quantity
L70101 kit
產品號碼 L7010
價格 (TWD)
14,800.00
Online offer
Ends: 31-Dec-2025
18,500.00
您節省 3,700.00 (20%)
Each
新增至購物車
Quantity:
1 kit
價格 (TWD)
14,800.00
Online offer
Ends: 31-Dec-2025
18,500.00
您節省 3,700.00 (20%)
Each
新增至購物車
The LIVE/DEAD® Cell-Mediated Cytotoxicity Kit measures natural killer (NK) cell-mediated, lymphokine-activated killer (LAK) cell-mediated and T cell mediated cytotoxicity. Target cells are preincubated with the green-fluorescent membrane stain DiOC18 and then mixed with effector cells in the presence of the red-fluorescent, membrane-impermeant dye, propidium iodide. Live and dead target cells retain the green-fluorescent membrane stain; target and effector cells with compromised membranes exhibit red-fluorescent nucleic acid staining; live effector cells are nonfluorescent.
For Research Use Only. Not for use in diagnostic procedures.
規格
Cell TypeMammalian Cells, Eukaryotic Cells
DescriptionLIVE/DEAD™ Cell-Mediated Cytotoxicity Kit, for animal cells
Detection MethodFluorescence
Dye TypeOther Label(s) or Dye(s)
FormatTube(s), Slide(s)
Quantity1 kit
Shipping ConditionRoom Temperature
ColorGreen, Red
Emission501/617
Excitation Wavelength Range484, 536 nm
For Use With (Application)Viability Assay
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Product LineLIVE/DEAD
Product TypeCell-Mediated Cytotoxicity Kit
Unit SizeEach
內容物與存放
Store in refrigerator at 2°C to 8°C and protect from light.

常見問答集 (常見問題)

Which optical filter sets are compatible with the LIVE/DEAD Cell-Mediated Cytotoxicity Kit?

The fluorescence from both live and dead bacteria stained with the LIVE/DEAD Cell-Mediated Cytotoxicity Kit may be viewed simultaneously with any standard fluorescein long-pass filter set. Alternatively, the fluorescent live and dead cells may be viewed separately with fluorescein and either rhodamine or Texas Red bandpass filter sets. A summary of the fluorescence microscope filter sets recommended for use with the LIVE/ DEAD Cell-Mediated Cytotoxicity Kit is shown in Table 1 on Page 2 in the manual (https://tools.thermofisher.com/content/sfs/manuals/mp07010.pdf).

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引用資料與參考文獻 (14)

引用資料與參考文獻
Abstract
Evaluation of human mast cell-mediated cytotoxicity by DIOC18 target cell labeling in flow cytometry.
Authors:Ozdemir O,
Journal:J Immunol Methods
PubMed ID:17188705
'(51)Cr release assay (CRA) is still the standard method to study mast cell (MC)-mediated cytotoxicity in vitro. Non-radioactive methods e.g. MTT, Hoechst 22147 staining, have also been used. Though CRA has the benefit of being reproducible, it has several drawbacks e.g. spontaneous release and radioactivity. The basic strategy of this ... More
Development of a lung slice preparation for recording ion channel activity in alveolar epithelial type I cells.
Authors:Bourke S, Mason HS, Borok Z, Kim KJ, Crandall ED, Kemp PJ,
Journal:Respir Res
PubMed ID:15857506
'Lung fluid balance in the healthy lung is dependent upon finely regulated vectorial transport of ions across the alveolar epithelium. Classically, the cellular locus of the major ion transport processes has been widely accepted to be the alveolar type II cell. Although evidence is now emerging to suggest that the ... More
Direct visualisation and quantification of cellular cytotoxicity using two colour flourescence.
Authors:Kroesen BJ, Mesander G, ter Haar JG, The TH, de Leij L
Journal:J Immunol Methods
PubMed ID:1431162
A fluorescence method is described for the evaluation of cell death induced by cellular cytolytic activity. A green fluorescent membrane dye, D275, was used to label various target cell lines and propidium iodide (PI) uptake was used to assay cell death. Natural killer (NK), lymphokine activated killer (LAK) as well ... More
Rapid flow cytometric assay for the assessment of natural killer cell activity.
Authors:Chang L, Gusewitch GA, Chritton DB, Folz JC, Lebeck LK, Nehlsen-Cannarella SL
Journal:J Immunol Methods
PubMed ID:8228287
A new assay using flow cytometry has been established to assess natural killer (NK) lytic activity with common bench top instrumentation. This assay uses a cyanine membrane dye to stain live K562 target cells and an iodide nuclear dye to evaluate dead cells, and provides a method of reliably separating ... More
Human Vγ2Vδ2 T cells limit breast cancer growth by modulating cell survival-, apoptosis-related molecules and microenvironment in tumors.
Authors:
Journal:Int J Cancer
PubMed ID:23595559
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