ProLong™ Gold Antifade Mountant with DNA Stain DAPI

引用資料與參考文獻 (30)

Invitrogen™

ProLong™ Gold Antifade Mountant with DNA Stain DAPI

Name: ProLong™ Gold Antifade Mountant with DNA Stain DAPI
SKU: P36935
Price: 9450.00 TWD

ProLong Gold Antifade Mountant with DNA Stain DAPI come mixed and ready-to-use to make counterstaining more convenient and consistent while protecting fluorescently stained samples.

變更視圖

產品號碼	Quantity
P36935	5 x 2 mL
P36941	1 x 2 mL
P36931	1 x 10 mL

3 選項

產品號碼 P36935

價格 (TWD)

9,450.00

Online offer

Ends: 31-Dec-2025

12,600.00

您節省 3,150.00 (25%)

Each

新增至購物車

Quantity:

5 x 2 mL

價格 (TWD)

9,450.00

Online offer

Ends: 31-Dec-2025

12,600.00

您節省 3,150.00 (25%)

Each

新增至購物車

ProLong Gold Antifade Mountant with DNA Stain DAPI is a mounting media that can be directly applied to fluorescently labeled cell or tissue samples on microscope slides. It comes ready-to-use and stored at room temperature—just apply a drop, add a coverslip, cure, and image. ProLong Gold Antifade Mountants come in a 2-mL dropper bottle or 10-mL bottle. It is formulated with the blue DNA stain DAPI to make your nuclear counterstaining more convenient and consistent when preparing your samples for imaging.

ProLong Gold Antifade Mountant with DNA Stain DAPI contains chemical components designed to protect fluorescent dyes from fading (photobleaching) during fluorescence microscopy experiments. It is a curing mounting media that forms an optical path of 1.47 RI and allows longer-term storage of the sample.

Many mountants can quench initial fluorescence signal strength by up to 80%. ProLong Gold Antifade Mountant was developed to minimize this initial signal quenching for the broadest range of colors and dyes and is formulated with the DAPI DNA nuclear stain to simplify your experiments. DAPI stain is excited by UV light at 360 nm when bound to DNA, with an emission maximum at 460 nm, and is detected using a DAPI traditional filter.

ProLong Gold Antifade Mountant is not recommended for mounting samples containing fluorescent proteins such as GFP or TagRFP but can be used with Alexa Fluor dyes of your choice. For superior antifade protection of fluorescent proteins and fluorescent dyes, ProLong Diamond or ProLong Glass Antifade Mountants are recommended. ProLong Glass mountants are recommended for all immersion oil imaging applications. For immediate viewing of a sample, choose our non-curing mountant, SlowFade Gold Antifade Mountant with DAPI.

For Research Use Only. Not for use in diagnostic procedures.

規格

Green FeaturesLess hazardous, Sustainable packaging

Quantity5 x 2 mL

Shipping ConditionRoom Temperature

Product LineProLong

Product TypeAntifade Mountant

Reagent TypeMounting Solution, Antifade Solution

Volume (Metric)2 mL

Unit SizeEach

內容物與存放

Storage at room temperature is recommended but can also be stored frozen (-5 to -30°C). Protect from light.

常見問答集 (常見問題)

What is the difference between ProLong and SlowFade antifade reagents?

Our ProLong antifade reagents dispense as a liquid that will solidify upon the evaporation of water. SlowFade antifade reagents remain liquid. If you are going to image right away and then dispose of your sample, you do not need a mountant that hardens, such as the SlowFade reagents. If you wish to archive your slide for more than a day, you will want a mounting medium that hardens (or “cures”). This hardening will limit the off-rates of various dye-conjugated antibodies and provides a better refractive index. Also, there will be a lower diffusion rate of free radicals, limiting photobleaching.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

If I use ProLong Gold Antifade Mountant to mount my slides, should I seal the edges?

ProLong Gold Antifade Mountant hardens overnight at room temperature. For short-term storage (a couple of weeks) you do not need to seal the edges of the coverslip, and the sample should be stable. Beyond that time, though, some dye conjugates will have an off-rate into the medium, so cold storage is recommended. Sealing the edges will prevent long-term discoloration (golden color) from developing around the edges of the coverslip as the anti-oxidants oxidize.

The edges may be sealed with melted paraffin, VALAP (1:1:1 vaseline, lanolin, paraffin) or epoxy glue. Nail polish is not recommended as various components of nail polish may diffuse into the mountant and quench fluorescent dyes.

Find additional tips, troubleshooting help, and resources within our Cell Imaging Support Center.

Some antifade mounting media stay as liquid whereas others harden. What is the benefit of having one that hardens?

If you are going to image right away and then dispose of your sample, you probably want a mountant that does not harden. If you wish to archive your slide for more than a day, you want a mountant that hardens (or "cures"). This hardening will slow or prevent off-rate of your dye or conjugate and often produces a better refractive index. Secondary sealing is usually not necessary. Also there will be lower diffusion of free radicals, thus limiting photobleaching.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I mounted my cells in ProLong antifade mounting medium, but now I want to go back and re-label them. Is there a way I can unmount the coverslip after it has cured (hardened)?

Yes. Put the slide in a Coplin jar or beaker filled with warm (37^oC) PBS buffer and let it sit, no agitation is required. The hardened ProLong mountant will swell and may slide off or be easily dislodged. If cells are adherent to the coverslip, make certain the coverslip side containing the cell or tissue sample does not land face down in the container or become scratched upon handling. Remove the coverslip, wash a couple of times, and proceed with re-staining and re-mounting in new ProLong mountant.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am using ProLong antifade mounting medium. Do I need to let it cure before imaging? Do I need to seal the edges of the coverslip?

You can image before it cures (hardens), and it will still slow photobleaching, but you have to let it cure overnight to get the best refractive index (resolution). There is no need to seal the edges. In fact, if you seal before it cures, it won't cure correctly. If you are archiving the slide for more than a month, though, seal the edges with resin, paraffin or VALAP (1:1:1 vaseline, lanolin, paraffin) after it cures or there may be slight discoloration along the edges over time.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all ProLong™ Gold Antifade Mountant with DNA Stain DAPI FAQs

引用資料與參考文獻 (30)

引用資料與參考文獻

Abstract

Super-resolution imaging reveals three-dimensional folding dynamics of the ÃŸ-globin locus upon gene activation.

Authors:van de Corput MP, de Boer E, Knoch TA, van Cappellen WA, Quintanilla A, Ferrand L, Grosveld FG,

Journal:J Cell Sci

PubMed ID:22767512

The chromatin architecture is constantly changing because of cellular processes such as proliferation, differentiation and changes in the expression profile during gene activation or silencing. Unravelling the changes that occur in the chromatin structure during these processes has been a topic of interest for many years. It is known that ... More

Resolution of de novo HIV production and trafficking in immature dendritic cells.

Authors:Turville SG, Aravantinou M, Stössel H, Romani N, Robbiani M,

Journal:Nat Methods

PubMed ID:18059278

'The challenge in observing de novo virus production in human immunodeficiency virus (HIV)-infected dendritic cells (DCs) is the lack of resolution between cytosolic immature and endocytic mature HIV gag protein. To track HIV production, we developed an infectious HIV construct bearing a diothiol-resistant tetracysteine motif (dTCM) at the C terminus ... More

Androgen induces expression of the multidrug resistance protein gene MRP4 in prostate cancer cells.

Authors:Cai C, Omwancha J, Hsieh CL, Shemshedini L

Journal:Prostate Cancer Prostatic Dis

PubMed ID:17003774

'Multidrug resistance-associated proteins (MRPs) may mediate multidrug resistance in tumor cells. Using a gene array analysis, we have identified MRP4 as an androgen receptor (AR)-regulated gene. Dihydrotestosterone induced MRP4 expression in both androgen-dependent and -independent LNCaP cells, whereas there was little detectable expression in PC-3 or normal prostate epithelial cells. ... More

H2AX phosphorylation within the G1 phase after UV irradiation depends on nucleotide excision repair and not DNA double-strand breaks.

Authors:Marti TM, Hefner E, Feeney L, Natale V, Cleaver JE

Journal:Proc Natl Acad Sci U S A

PubMed ID:16788066

'The variant histone H2AX is phosphorylated in response to UV irradiation of primary human fibroblasts in a complex fashion that is radically different from that commonly reported after DNA double-strand breaks. H2AX phosphorylation after exposure to ionizing radiation produces foci, which are detectable by immunofluorescence microscopy and have been adopted ... More

AZD2171: a highly potent, orally bioavailable, vascular endothelial growth factor receptor-2 tyrosine kinase inhibitor for the treatment of cancer.

Authors:Wedge SR, Kendrew J, Hennequin LF, Valentine PJ, Barry ST, Brave SR, Smith NR, James NH, Dukes M, Curwen JO, Chester R, Jackson JA, Boffey SJ, Kilburn LL, Barnett S, Richmond GH, Wadsworth PF, Walker M, Bigley AL, Taylor ST, Cooper L, Beck S, Jürgensmeier JM, Ogilvie DJ,

Journal:Cancer Res

PubMed ID:15899831

'Inhibition of vascular endothelial growth factor-A (VEGF) signaling is a promising therapeutic approach that aims to stabilize the progression of solid malignancies by abrogating tumor-induced angiogenesis. This may be accomplished by inhibiting the kinase activity of VEGF receptor-2 (KDR), which has a key role in mediating VEGF-induced responses. The novel ... More

View all ProLong™ Gold Antifade Mountant with DNA Stain DAPI citations