Quant-iT™ Protein Assay Kit
Quant-iT™ Protein Assay Kit
Quant-iT™ Protein Assay Kit
Quant-iT™ Protein Assay Kit
Quant-iT™ Protein Assay Kit
Quant-iT™ Protein Assay Kit
引用資料與參考文獻 (6)
Invitrogen™

Quant-iT™ Protein Assay Kit

The Quant-iT Protein Assay Kit makes protein quantitation easy and accurate. The kit provides concentrated assay reagent, dilution buffer, and深入閱讀
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產品號碼Quantity
Q332101 Kit
產品號碼 Q33210
價格 (TWD)
15,330.00
Online offer
Ends: 31-Dec-2025
21,900.00
您節省 6,570.00 (30%)
Each
新增至購物車
Quantity:
1 Kit
Request bulk or custom format
價格 (TWD)
15,330.00
Online offer
Ends: 31-Dec-2025
21,900.00
您節省 6,570.00 (30%)
Each
新增至購物車
The Quant-iT Protein Assay Kit makes protein quantitation easy and accurate. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted BSA standards. Simply dilute the reagent, load it into the wells of a microplate, add 1-20 μL of sample, mix, then measure the fluorescence. The assay is highly selective for protein and exhibits very little protein-to-protein variation. The assay is performed at room temperature, and the signal is stable for 3 hours. Common contaminants, such as salts, solvents, or DNA—but not detergents—are well tolerated in the assay. Quant-iT DNA Assay Kits (Q33120, Q33130) and a Quant-iT RNA Assay Kit (Q33140) are also available.
For Research Use Only. Not for use in diagnostic procedures.
規格
AssayFluorescent Protein Assay
For Use With (Equipment)Microplate Reader
Gene ID (Entrez)3919448
Kit ContentsConcentrated assay reagent, dilution buffer, and pre-diluted BSA standards
Product LineQuant-iT
Product TypeProtein Quantitation Assay
Quantity1 Kit
Sample Volume1 to 20 uL
Shipping ConditionRoom Temperature
Storage RequirementsStore in refrigerator (2° to 8°C). Protect from light.
Detection MethodFluorescence
Unit SizeEach
內容物與存放
Store in refrigerator (2–8°C) and protect from light.

常見問答集 (常見問題)

Why am I getting negative fluorescence values with my Qubit Assays?

Negative fluorescence is a physical impossibility. It is an artifact from software autocorrecting for background signal. This means your reader is picking up and subtracting out background light at the cost of your data. Make sure to do a buffer-only control and assess the type of signal. You may need to switch to a different plate.

I have a Quant-iT DNA Kit and want to use it for the Qubit Fluorometer. Can I?

Yes, the manual has directions for this application. You will use the 0 ng/µL lambda dsDNA HS standard to generate Standard #1. You will prepare a dilution of the 10 ng/µL lambda dsDNA HS standard to generate Standard #2. You then prepare the samples and compare them to this 2-point standard curve. The Quant-iT dsDNA BR Kit can be used in a similar manner.

What is the useful pH range for Quant-iT DNA kits?

The buffer included in the kit should assure the proper pH range, even if your DNA is at a pH outside of this range, since at least a 10-fold excess of kit buffer over sample is used in the assay.

I'm trying to quantify some DNA labeled with a fluorophore. Will this work?

PicoGreen dye and other fluorescence-based quantification reagents are not recommended for quantifying dye-conjugated nucleic acids. The attached dye molecules can interfere with either binding and/or fluorescence output of the quantification reagents.

Does DNA length have an effect on the dsDNA assays?

Strands that are roughly in the 20-mer range or shorter show a lower level of signal. For dsDNA samples that are composed of mostly short strands, the reagent may still be used, but one should use a dsDNA standard that is of comparable length as the sample.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

View all Quant-iT™ Protein Assay Kit FAQs

引用資料與參考文獻 (6)

引用資料與參考文獻
Abstract
Acrolein inhibits cytokine gene expression by alkylating cysteine and arginine residues in the NF-kappaB1 DNA binding domain.
Authors:Lambert C, Li J, Jonscher K, Yang TC, Reigan P, Quintana M, Harvey J, Freed BM,
Journal:J Biol Chem
PubMed ID:17491020
Cigarette smoke is a potent inhibitor of pulmonary T cell responses, resulting in decreased immune surveillance and an increased incidence of respiratory tract infections. The alpha,beta-unsaturated aldehydes in cigarette smoke (acrolein and crotonaldehyde) inhibited production of interleukin-2 (IL-2), IL-10, granulocyte-macrophage colony-stimulating factor, interferon-gamma, and tumor necrosis factor-alpha by human T ... More
Functional selection of phagocytosis-promoting genes: cell sorting-based selection.
Authors:Jeon H, Go Y, Seo M, Lee WH, Suk K,
Journal:J Biomol Screen
PubMed ID:20660795
Phagocytosis is a critical host defense mechanism that clears invading pathogens, apoptotic cells, and cell debris; it is an essential process for normal development, tissue remodeling, immune response, and inflammation. Here, a functional selection strategy was used to isolate novel phagocytosis-promoting genes. After the retroviral transfer of mouse brain cDNA ... More
Quantitation of protein.
Authors:Noble JE, Bailey MJ,
Journal:Methods Enzymol
PubMed ID:19892168
The measurement of protein concentration in an aqueous sample is an important assay in biochemistry research and development labs for applications ranging from enzymatic studies to providing data for biopharmaceutical lot release. Spectrophotometric protein quantitation assays are methods that use UV and visible spectroscopy to rapidly determine the concentration of ... More
A comparison of protein quantitation assays for biopharmaceutical applications.
Authors:Noble JE, Knight AE, Reason AJ, Di Matola A, Bailey MJ,
Journal:Mol Biotechnol
PubMed ID:17914170
Dye-based protein determination assays are widely used to estimate protein concentration, however various reports suggest that the response is dependent on the composition and sequence of the protein, limiting confidence in the resulting concentration estimates. In this study a diverse set of model proteins representing various sizes of protein and ... More
Targeted quantitation of overexpressed and endogenous cystic fibrosis transmembrane conductance regulator using multiple reaction monitoring tandem mass spectrometry and oxygen stable isotope dilution.
Authors:Jiang H, Ramos AA, Yao X,
Journal:Anal Chem
PubMed ID:19947594
Cystic fibrosis transmembrane conductance regulator (CFTR) functions as an ion channel in the apical plasma membrane of epithelial cells. Mutations in the gene coding for CFTR cause cystic fibrosis (CF). A major cellular dysfunction is insufficient apical plasma membrane expression of the protein. Its correction is important for developing new ... More
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