dNTP Mix (10 mM each)
dNTP Mix (10 mM each)
Thermo Scientific™

dNTP Mix (10 mM each)

Thermo Scientific dNTP Mix (10 mM) contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 10 mM.
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產品號碼Quantity
R01910.2 mL
R01921 mL
R01935 x 1 mL
R019410 x 1 mL
產品號碼 R0191
價格 (TWD)
1,190.00
Online offer
Ends: 31-Dec-2025
1,690.00
您節省 500.00 (30%)
Each
新增至購物車
Quantity:
0.2 mL
Request bulk or custom format
價格 (TWD)
1,190.00
Online offer
Ends: 31-Dec-2025
1,690.00
您節省 500.00 (30%)
Each
新增至購物車

Thermo Scientific dNTP Mix (10 mM) contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 10 mM. The nucleotides have greater than 99% purity, are free of nuclease activities, human and E. coli DNA. Mixes offer the ability to reduce the number of pipetting steps and the associated risk of reaction set up errors, and are designed for use in a wide variety molecular biology applications.

Features

  • Greater than 99% purity confirmed by HPLC
  • Free of human and E. coli DNA
  • Long term stability, even after multiple freeze-thaw cycles when properly stored
  • Up to 95% of dNTPs remain in triphosphate form after 7 weeks at room temperature
  • Up to 90% of dNTPs remain in triphosphate form after 30 cycles of PCR (1 minute at 94°C, 3 minutes at 72°C)

Applications

  • Long range PCR (40 kb)
  • cDNA synthesis
  • PCR, RT-PCR, qPCR
  • High fidelity PCR
  • DNA labeling and sequencing
  • LAMP, RDA, MDA
For Research Use Only. Not for use in diagnostic procedures.
規格
Purity or Quality Grade>99%
Quantity0.2 mL
Shipping ConditionDry Ice
Concentration10 mM
For Use With (Application)Standard PCR, RT-PCR, Real Time PCR (qPCR)
FormLiquid
Label or DyeUnlabeled
Unit SizeEach
內容物與存放
dNTP Mix (10 mM) contains premixed dATP, dCTP, dGTP and dTTP at a final concentration of 10 mM (0.2 mL)

Store at –20°C.

常見問答集 (常見問題)

What is the concentration of individual dNTPs in the Thermo Scientific dNTP Mix (Cat. No. R0191)?

The Thermo Scientific dNTP Mix (Cat. No. R0191) contains premixed aqueous solutions of dATP, dCTP, dGTP, and dTTP, each at a final concentration of 10 mM.

What concentration of dNTPs is typically used in a PCR amplification?

The four dNTPs (dATP, dCTP, dTTP, dGTP) are used at a final concentration of 200 µM each or a total of 0.8 mM in standard amplifications. For PCR amplifications that require high fidelity, lower dNTP concentrations (on the order of 10-50 µM) work optimally.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.