NET Test

Use Thermo Scientific™ Remel™ NET Test for the rapid, presumptive identification of Neisseria gonorrhoeae, Neisseria meningitidis, Neisseria lactamica and Moraxella catarrhalis. In 1978, D'Amato et al. described a method to identify N. gonorrhoeae and N. meningitidis using chromogenic substrates to determine enzyme profiles¹. Hoke et al. used modification of his technique to characterize atypical strains of N. gonorrhoeae and N. meningitidis². In 1982, Yajko et al. described a method to identify Neisseria spp. using mixed chromogenic substrates³.

NET employs three synthetic chromogenic substrates to detect preformed enzymes. The enzyme β-D-galactopyranoside forms a blue colored complex in the presence of oxygen and γ-glutamyl peptidase hydrolyzes γ-glutamyl-ρ-nitroanilide to form a yellow color. The third enzyme, prolylaminopeptidase, hydrolyzes L-proline β-naphthylamide which is detected by a color change to pink-red after adding ρ-dimethyl-aminocinnamaldehyde.

• Ready to use – Prepared reagents.
• Easy to store – Store in its original container at room temperature (2-8°C) until further use.

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