Ni-NTA Agarose
Ni-NTA Agarose
Invitrogen™

Ni-NTA Agarose

Ni-NTA Agarose is a nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence.深入閱讀
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產品號碼Quantity
R9011525 mL
R9010110 mL
R90110100 mL
產品號碼 R90115
價格 (TWD)
14,070.00
Online offer
Ends: 31-Mar-2026
20,100.00
您節省 6,030.00 (30%)
Each
新增至購物車
Quantity:
25 mL
Request bulk or custom format
價格 (TWD)
14,070.00
Online offer
Ends: 31-Mar-2026
20,100.00
您節省 6,030.00 (30%)
Each
新增至購物車
Ni-NTA Agarose is a nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence. Proteins bound to the resin may be eluted with either low pH buffer or by competition with imidazole or histidine. One-step purification can be performed under both native and denaturing conditions. Ni-NTA uses the chelating ligand nitrilotriacetic acid (NTA) coupled to a cross-linked 6% agarose resin that is suitable for use in batch and gravity flow applications.
For Research Use Only. Not for use in diagnostic procedures.
規格
Quantity25 mL
Stationary PhaseNi-NTA
Column TypeAffinity Column
FormSuspension
TypeAgarose
Unit SizeEach
內容物與存放
The Ni-NTA resin is pre-charged and able to bind up to 50 mg of recombinant protein per 1 ml of resin. It is provided as a 50% slurry in 30% ethanol. The resin will appear blue in color when charged with Ni2+ . Store at +4°C. Ni-NTA resin is guaranteed stable for 6 months when properly stored.

常見問答集 (常見問題)

Can ProBond or Ni-NTA beads be used for large-scale preparations?

ProBond and Ni-NTA beads can be used in FPLC columns. However, the beads can only withstand low pressure (~43.5 psi max).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What should the typical protein recovery be when using the Probond Purification System or Ni-NTA Purification system?

Both systems are qualified by purifying 2 mg of myoglobin protein on a column and performing a Bradford assay. Protein recovery must be 75% or higher.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.