SYTO™ 84 Orange Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO
SYTO™ 84 Orange Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO
引用資料與參考文獻 (1)
Invitrogen™

SYTO™ 84 Orange Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO

The cell-permeant SYTO 84 orange fluorescent nucleic acid stain exhibits bright, orange fluorescence upon binding to nucleic acids. Because the深入閱讀
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產品號碼Quantity
S11365250 μL
產品號碼 S11365
價格 (TWD)
11,200.00
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Ends: 31-Mar-2026
16,000.00
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Quantity:
250 μL
價格 (TWD)
11,200.00
Online offer
Ends: 31-Mar-2026
16,000.00
您節省 4,800.00 (30%)
Each
新增至購物車

The cell-permeant SYTO 84 orange fluorescent nucleic acid stain exhibits bright, orange fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Orange Fluorescent Nucleic Acid Stain Sampler Kit (Cat. No. S-11360) to enable researchers to find the most appropriate orange-fluorescent SYTO stain for their system.

Any physiological buffer between pH 7.0–8.0, including PBS, can be used to dilute the SYTO dyes for the staining solution.

For Research Use Only. Not for use in diagnostic procedures.
規格
ColorOrange
DescriptionSYTO™ 84 Orange Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO
Detection MethodFluorescence
Dye TypeCell-Permeant
Emission582 nm
Excitation Wavelength Range567 nm
For Use With (Equipment)Fluorescence Microscope
Product LineSYTO
Quantity250 μL
Shipping ConditionRoom Temperature
Volume (Metric)250 μL
Label TypeFluorescent Dye
Product TypeNucleic Acid Stain
SubCellular LocalizationNucleic Acids
Unit SizeEach
內容物與存放
Store in freezer at -5°C to -30°C and protect from light.

常見問答集 (常見問題)

How do SYTO dyes bind to DNA?

The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:

1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid. 4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).

SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用資料與參考文獻 (1)

引用資料與參考文獻
Abstract
Multiparameter assessments to determine the effects of sugars and antimicrobials on a polymicrobial oral biofilm.
Authors:Yang Y, Sreenivasan PK, Subramanyam R, Cummins D,
Journal:Appl Environ Microbiol
PubMed ID:17021225
Clinical studies indicate relationships between dental plaque, a naturally formed biofilm, and oral diseases. The crucial role of nonmicrobial biofilm constituents in maintaining biofilm structure and biofilm-specific attributes, such as resistance to shear and viscoelasticity, is increasingly recognized. Concurrent analyses of the diverse nonmicrobial biofilm components for multiparameter assessments formed ... More