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引用資料與參考文獻 (7)
Invitrogen™
Sulfate Latex Beads, 4% w/v, 5 μm
Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from深入閱讀
| 產品號碼 | Quantity |
|---|---|
| S37227 | 15 mL |
產品號碼 S37227
價格 (TWD)
***
Quantity:
15 mL
價格 (TWD)
***
Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Because of the way that the polystyrene chains arrange themselves in the bead, the surface is very hydrophobic in character, making these ideal materials for the adsorption of materials such as proteins.
These sulfate latex microspheres are stabilized by sulfate charges. In some cases, hydroxyl and carboxyl groups are also present in small amounts bound to the polymer chains. The pKa of the sulfate group is <2 and consequently the particles are stable in acidic media. Suspensions of the particles are stable up to almost 0.30 M univalent electrolyte concentrations and therefore may be used in media of physiological ionic strength. However they are hydrophobic particles and will undergo aggregation in the presence of low concentrations of divalent or trivalent cations unless stabilized by a hydrophilic coating. Sulfate latex is suitable for calibration of particle size analysis equipment and are also appropriate for use in diagnostic test systems which rely upon physical adsorption of antigens or antibodies.
Microspheres Are Available in Many Sizes and Surface Functionalities
• 25 sizes to choose from—0.02 microns to 16 microns
• 20 different surface—for ready conjugation to virtually any material
• Surfactant free—ultraclean
• Various quantities—from milliliter to 500-liter amounts
Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)
Get More Information
Read a technical overview of microspheres and find out about high activity beads, super active beads, specialty beads, and review bead-coupling protocols on the IDC Surfactant-free Latex Beads application page.
We’ll Make a Custom Microsphere Product for You
If you don’t see the microsphere you’re looking for in our stocked list, we’ll make a custom microsphere for you. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 13485:2000 certified.
For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.
These sulfate latex microspheres are stabilized by sulfate charges. In some cases, hydroxyl and carboxyl groups are also present in small amounts bound to the polymer chains. The pKa of the sulfate group is <2 and consequently the particles are stable in acidic media. Suspensions of the particles are stable up to almost 0.30 M univalent electrolyte concentrations and therefore may be used in media of physiological ionic strength. However they are hydrophobic particles and will undergo aggregation in the presence of low concentrations of divalent or trivalent cations unless stabilized by a hydrophilic coating. Sulfate latex is suitable for calibration of particle size analysis equipment and are also appropriate for use in diagnostic test systems which rely upon physical adsorption of antigens or antibodies.
Microspheres Are Available in Many Sizes and Surface Functionalities
• 25 sizes to choose from—0.02 microns to 16 microns
• 20 different surface—for ready conjugation to virtually any material
• Surfactant free—ultraclean
• Various quantities—from milliliter to 500-liter amounts
Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)
Get More Information
Read a technical overview of microspheres and find out about high activity beads, super active beads, specialty beads, and review bead-coupling protocols on the IDC Surfactant-free Latex Beads application page.
We’ll Make a Custom Microsphere Product for You
If you don’t see the microsphere you’re looking for in our stocked list, we’ll make a custom microsphere for you. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 13485:2000 certified.
For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
規格
Product LineIDC
Quantity15 mL
Shipping ConditionRoom Temperature
Surface ModificationSulfate
Diameter (Metric)5 μm
MaterialPolystyrene
Product TypeLatex Beads
Unit SizeEach
內容物與存放
Store in refrigerator (2–8°C).
引用資料與參考文獻 (7)
引用資料與參考文獻
Abstract
Synthetic CD4+ T cell-targeted antigen-presenting cells elicit protective antitumor responses.
Journal:Cancer Res
PubMed ID:18413771
CD4(+) helper T cells are critical for protective immune responses and yet suboptimally primed in response to tumors. Cell-based vaccination strategies are under evaluation in clinical trials but limited by the need to derive antigen-presenting cells (APC) from patients or compatible healthy donors. To overcome these limitations, we developed CD4(+)
Stored Fas ligand, a mediator of rapid CTL-mediated killing, has a lower threshold for response than degranulation or newly synthesized Fas ligand.
Journal:J Immunol
PubMed ID:19949069
'CTL lyse target cells through the release of cytolytic granule mediators and expression of the death receptor ligand Fas ligand (FasL). We previously demonstrated that FasL is stored in vesicles distinct from cytolytic granules and is translocated to the cell surface within 15 min of TCR stimulation, followed by a
Antigen-experienced CD4(+) T cells limit naïve T-cell priming in response to therapeutic vaccination in vivo.
Journal:Cancer Res
PubMed ID:20631073
'CD4(+) T cells play a central role in protective immunity. In a mouse tumor model, we previously found that tumor growth elicits natural CD4(+) T-cell responses, but impedes therapeutic vaccination. We show here that inhibition of vaccine-mediated naïve T-cell priming is due to the presence of a minor but distinct
Chromogranin A regulates tumor self-seeding and dissemination.
Journal:Cancer Res
PubMed ID:22139379
Cancer progression involves the seeding of malignant cells in circulation and the colonization of distant organs. However, circulating neoplastic cells can also reinfiltrate the tumor of origin. This process, called
Ezrin and moesin function together to promote T cell activation.
Journal:J Immunol
PubMed ID:19124745
The highly homologous proteins ezrin, radixin, and moesin link proteins to the actin cytoskeleton. The two family members expressed in T cells, ezrin and moesin, are implicated in promoting T cell activation and polarity. To elucidate the contributions of ezrin and moesin, we conducted a systematic analysis of their function